B6Brd;B6N-Tyr^c-Brd Ltbp1^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:05861
Citation information	RRID:IMSR_EM:05861 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6Brd;B6N-Tyr^c-Brd Ltbp1^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0127_1_A03
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Ltbp1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Ltbp1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0127_1_A03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6NTac males
Breeding at archiving centre	Received as frozen sperm. Due to the possible presence of C57BL/6Brd-Tyr^c-Brd, albino pups might be produced by intercrossing.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal recessive cutis laxa type 1 / Orphanet_90349

IMPC phenotypes (allele matching)

abnormal cranium morphology / IMPC
abnormal snout morphology / IMPC
abnormal radius morphology / IMPC
decreased body length / IMPC
decreased body weight / IMPC
abnormal coat/hair pigmentation / IMPC
abnormal tooth morphology / IMPC
vertebral fusion / IMPC
abnormal ulna morphology / IMPC
increased circulating bilirubin level / IMPC
abnormal incisor morphology / IMPC
increased hemoglobin content / IMPC
decreased total body fat amount / IMPC
decreased bone mineral content / IMPC
lipid deposition / IMPC

IMPC phenotypes (gene matching)

decreased body weight / IMPC
decreased body length / IMPC
lipid deposition / IMPC
abnormal snout morphology / IMPC
increased hemoglobin content / IMPC
abnormal coat/hair pigmentation / IMPC
decreased total body fat amount / IMPC
abnormal ulna morphology / IMPC
vertebral fusion / IMPC
abnormal radius morphology / IMPC
increased circulating bilirubin level / IMPC
abnormal tooth morphology / IMPC
abnormal cranium morphology / IMPC
abnormal incisor morphology / IMPC
decreased bone mineral content / IMPC

MGI phenotypes (allele matching)

increased leukocyte cell number / MGI
decreased circulating LDL cholesterol level / MGI
decreased circulating HDL cholesterol level / MGI
abnormal cranium morphology / MGI
abnormal snout morphology / MGI
abnormal radius morphology / MGI
decreased body length / MGI
decreased body weight / MGI
abnormal tooth morphology / MGI
decreased circulating alanine transaminase level / MGI
decreased circulating iron level / MGI
abnormal ulna morphology / MGI
decreased circulating cholesterol level / MGI
abnormal incisor morphology / MGI
decreased circulating serum albumin level / MGI
increased mean corpuscular hemoglobin / MGI
increased hemoglobin content / MGI
increased blood urea nitrogen level / MGI
decreased circulating total protein level / MGI
decreased circulating aspartate transaminase level / MGI
decreased total body fat amount / MGI
decreased bone mineral content / MGI

MGI phenotypes (gene matching)

short mandible / MGI
short maxilla / MGI
decreased circulating LDL cholesterol level / MGI
decreased circulating HDL cholesterol level / MGI
increased leukocyte cell number / MGI
double outlet right ventricle / MGI
abnormal cranium morphology / MGI
abnormal snout morphology / MGI
short snout / MGI
abnormal jaw morphology / MGI
abnormal radius morphology / MGI
decreased body length / MGI
decreased body weight / MGI
cyanosis / MGI
abnormal tooth morphology / MGI
persistent truncus arteriosis / MGI
dilated renal tubules / MGI
decreased circulating alanine transaminase level / MGI
liver fibrosis / MGI
abnormal coronary artery morphology / MGI
decreased circulating iron level / MGI
interrupted aortic arch / MGI
right aortic arch / MGI
small basioccipital bone / MGI
small basisphenoid bone / MGI
abnormal ulna morphology / MGI
decreased circulating cholesterol level / MGI
abnormal incisor morphology / MGI
decreased circulating serum albumin level / MGI
increased mean corpuscular hemoglobin / MGI
increased hemoglobin content / MGI
increased blood urea nitrogen level / MGI
decreased circulating total protein level / MGI
abnormal vascular smooth muscle morphology / MGI
decreased circulating aspartate transaminase level / MGI
abnormal fourth pharyngeal arch artery morphology / MGI
polycystic kidney / MGI
decreased total body fat amount / MGI
decreased bone mineral content / MGI
common atrium / MGI
perimembraneous ventricular septal defect / MGI
abnormal truncus arteriosus septation / MGI
vascular ring / MGI
abnormal ascending aorta and coronary artery attachment / MGI
integument phenotype / MGI
neonatal lethality, complete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
renal glomerulus cysts / MGI
abnormal cardiac neural crest cell morphology / MGI
abnormal lower incisor morphology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Ltbp1tm1a(EUCOMM)Wtsi/WtsiH