C57BL/6NTac-Sar1b^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:05863
Citation information	RRID:IMSR_EM:05863 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6NTac-Sar1b^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0113_3_C11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Sar1b^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Sar1b
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0113_3_C11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6NTac males
Breeding at archiving centre	Frozen material received.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Chylomicron retention disease / Orphanet_71

IMPC phenotypes (allele matching)

decreased circulating LDL cholesterol level / IMPC
decreased circulating HDL cholesterol level / IMPC
abnormal skin condition / IMPC
decreased circulating alanine transaminase level / IMPC
decreased circulating cholesterol level / IMPC
decreased circulating serum albumin level / IMPC
thrombocytosis / IMPC
decreased circulating aspartate transaminase level / IMPC
preweaning lethality, complete penetrance / IMPC
hyperplasia / IMPC
lipid deposition / IMPC
extramedullary hemopoiesis / IMPC

IMPC phenotypes (gene matching)

hyperplasia / IMPC
decreased circulating aspartate transaminase level / IMPC
lipid deposition / IMPC
thrombocytosis / IMPC
extramedullary hemopoiesis / IMPC
decreased circulating HDL cholesterol level / IMPC
decreased circulating cholesterol level / IMPC
abnormal skin condition / IMPC
decreased circulating serum albumin level / IMPC
decreased circulating alanine transaminase level / IMPC
decreased circulating LDL cholesterol level / IMPC
preweaning lethality, complete penetrance / IMPC

MGI phenotypes (allele matching)

decreased circulating LDL cholesterol level / MGI
decreased circulating HDL cholesterol level / MGI
abnormal skin condition / MGI
increased circulating free fatty acid level / MGI
decreased circulating free fatty acid level / MGI
decreased circulating alanine transaminase level / MGI
decreased circulating cholesterol level / MGI
increased mean corpuscular hemoglobin / MGI

MGI phenotypes (gene matching)

decreased circulating LDL cholesterol level / MGI
decreased circulating HDL cholesterol level / MGI
abnormal skin condition / MGI
increased circulating free fatty acid level / MGI
decreased circulating free fatty acid level / MGI
decreased circulating alanine transaminase level / MGI
decreased circulating cholesterol level / MGI
increased mean corpuscular hemoglobin / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Sar1btm1a(EUCOMM)Wtsi/WtsiH