C57BL/6N-A^tm1Brd Wnt3^{tm2a(EUCOMM)Wtsi}/WtsiCnbc

Status	Available to order
EMMA ID	EM:06106
Citation information	RRID:IMSR_EM:06106 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-A^tm1Brd Wnt3^{tm2a(EUCOMM)Wtsi}/WtsiCnbc
Alternative name	EPD0555_1_C05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Wnt3^{tm2a(EUCOMM)Wtsi}
Gene/Transgene symbol	Wnt3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0555_1_C05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Tetraamelia-multiple malformations syndrome / Orphanet_3301

IMPC phenotypes (allele matching)

preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC

MGI phenotypes (gene matching)

absent limbs / MGI
abnormal germ layer development / MGI
abnormal ectoderm development / MGI
absent mesoderm / MGI
failure of primitive streak formation / MGI
failure to gastrulate / MGI
abnormal embryo size / MGI
absent amniotic folds / MGI
abnormal extraembryonic endoderm formation / MGI
abnormal extraembryonic tissue morphology / MGI
no phenotypic analysis / MGI
absent allantois / MGI
abnormal embryonic epiblast morphology / MGI
disorganized embryonic tissue / MGI
absent amnion / MGI
abnormal rostral-caudal axis patterning / MGI
embryonic lethality during organogenesis / MGI
absent primitive node / MGI
absent chorion / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
absent visceral yolk sac / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Wnt3tm2a(EUCOMM)Wtsi/WtsiCnbc