STOCK Pawr^tm1Jmos/Cnbc

Status	Available to order
EMMA ID	EM:06218
Citation information	RRID:IMSR_EM:06218 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	STOCK Pawr^tm1Jmos/Cnbc
Alternative name	Pawr
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Pawr^tm1Jmos
Gene/Transgene symbol	Pawr

Information from provider

Provider	Manuel Serrano
Provider affiliation	Molecular Oncology, CNIO (Spanish National Cancer Research Centre)
Genetic information	Exons 1 and 2 were replaced with a neomycin resistance gene. Embryonic fibroblasts derived from mice homozygous for this allele did not express the protein.
Phenotypic information	Decreased apoptosis. Increased B and T cell proliferation. Increased lymphocyte cell number. Enlarged spleen. Spleen hyperplasia.
Breeding history	Chimeras were crossed with CD1 females. Heterozygous offspring were crossed among themselves to generate homozygous mutants.
References	Genetic inactivation of Par4 results in hyperactivation of NF-kappaB and impairment of JNK and p38.;Garcia-Cao Isabel, Lafuente María José, Criado Luis M, Diaz-Meco María Teresa, Serrano Manuel, Moscat Jorge, ;2003;EMBO reports;4;307-12; 12634851 Simultaneous inactivation of Par-4 and PTEN in vivo leads to synergistic NF-kappaB activation and invasive prostate carcinoma.;Fernandez-Marcos Pablo J, Abu-Baker Shadi, Joshi Jayashree, Galvez Anita, Castilla Elias A, Cañamero Marta, Collado Manuel, Saez Carmen, Moreno-Bueno Gema, Palacios Jose, Leitges Michael, Serrano Manuel, Moscat Jorge, Diaz-Meco Maria T, ;2009;Proceedings of the National Academy of Sciences of the United States of America;106;12962-7; 19470463 Nephrin deficiency activates NF-kappaB and promotes glomerular injury.;Hussain Sagair, Romio Leile, Saleem Moin, Mathieson Peter, Serrano Manuel, Moscat Jorge, Diaz-Meco Maria, Scambler Peter, Koziell Ania, ;2009;Journal of the American Society of Nephrology : JASN;20;1733-43; 19497968 Par-4 inhibits Akt and suppresses Ras-induced lung tumorigenesis.;Joshi Jayashree, Fernandez-Marcos Pablo J, Galvez Anita, Amanchy Ramars, Linares Juan F, Duran Angeles, Pathrose Peterson, Leitges Michael, Cañamero Marta, Collado Manuel, Salas Clara, Serrano Manuel, Moscat Jorge, Diaz-Meco Maria T, ;2008;The EMBO journal;27;2181-93; 18650932 Tumour-suppression activity of the proapoptotic regulator Par4.;García-Cao Isabel, Duran Angeles, Collado Manuel, Carrascosa Maria J, Martín-Caballero Juan, Flores Juana M, Diaz-Meco Maria T, Moscat Jorge, Serrano Manuel, ;2005;EMBO reports;6;577-83; 15877079 Crosstalk between PKCzeta and the IL4/Stat6 pathway during T-cell-mediated hepatitis.;Durán Angeles, Rodriguez Angelina, Martin Pilar, Serrano Manuel, Flores Juana Maria, Leitges Michael, Diaz-Meco María T, Moscat Jorge, ;2004;The EMBO journal;23;4595-605; 15526032 Regulation of mature T lymphocyte proliferation and differentiation by Par-4.;Lafuente María José, Martin Pilar, Garcia-Cao Isabel, Diaz-Meco María Teresa, Serrano Manuel, Moscat Jorge, ;2003;The EMBO journal;22;4689-98; 12970181
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	homozygous CD-1 outbred stock (syn.: outbr. CD-1 or CD1, Swiss CD-1 or CD1, ICR(CD-1), etc.) males, wild-type CD-1 outbred stock (syn.: outbr. CD-1 or CD1, Swiss CD-1 or CD1, ICR(CD-1), etc.) females
Stage of embryos	Morula

Disease and phenotype information

IMPC phenotypes (gene matching)

decreased locomotor activity / IMPC
preweaning lethality, incomplete penetrance / IMPC
decreased circulating creatinine level / IMPC

MGI phenotypes (allele matching)

enlarged spleen / MGI
spleen hyperplasia / MGI
increased lymphocyte cell number / MGI
increased B cell proliferation / MGI
increased T cell proliferation / MGI
abnormal T-helper 2 physiology / MGI
decreased sensitivity to induced cell death / MGI

MGI phenotypes (gene matching)

enlarged spleen / MGI
spleen hyperplasia / MGI
no abnormal phenotype detected / MGI
behavioral despair / MGI
increased lymphocyte cell number / MGI
increased B cell proliferation / MGI
increased T cell proliferation / MGI
behavior/neurological phenotype / MGI
abnormal T-helper 2 physiology / MGI
decreased sensitivity to induced cell death / MGI

Literature references

Genetic inactivation of Par4 results in hyperactivation of NF-kappaB and impairment of JNK and p38.;Garcia-Cao Isabel, Lafuente María José, Criado Luis M, Diaz-Meco María Teresa, Serrano Manuel, Moscat Jorge, ;2003;EMBO reports;4;307-12; 12634851
Simultaneous inactivation of Par-4 and PTEN in vivo leads to synergistic NF-kappaB activation and invasive prostate carcinoma.;Fernandez-Marcos Pablo J, Abu-Baker Shadi, Joshi Jayashree, Galvez Anita, Castilla Elias A, Cañamero Marta, Collado Manuel, Saez Carmen, Moreno-Bueno Gema, Palacios Jose, Leitges Michael, Serrano Manuel, Moscat Jorge, Diaz-Meco Maria T, ;2009;Proceedings of the National Academy of Sciences of the United States of America;106;12962-7; 19470463
Nephrin deficiency activates NF-kappaB and promotes glomerular injury.;Hussain Sagair, Romio Leile, Saleem Moin, Mathieson Peter, Serrano Manuel, Moscat Jorge, Diaz-Meco Maria, Scambler Peter, Koziell Ania, ;2009;Journal of the American Society of Nephrology : JASN;20;1733-43; 19497968
Par-4 inhibits Akt and suppresses Ras-induced lung tumorigenesis.;Joshi Jayashree, Fernandez-Marcos Pablo J, Galvez Anita, Amanchy Ramars, Linares Juan F, Duran Angeles, Pathrose Peterson, Leitges Michael, Cañamero Marta, Collado Manuel, Salas Clara, Serrano Manuel, Moscat Jorge, Diaz-Meco Maria T, ;2008;The EMBO journal;27;2181-93; 18650932
Tumour-suppression activity of the proapoptotic regulator Par4.;García-Cao Isabel, Duran Angeles, Collado Manuel, Carrascosa Maria J, Martín-Caballero Juan, Flores Juana M, Diaz-Meco Maria T, Moscat Jorge, Serrano Manuel, ;2005;EMBO reports;6;577-83; 15877079
Crosstalk between PKCzeta and the IL4/Stat6 pathway during T-cell-mediated hepatitis.;Durán Angeles, Rodriguez Angelina, Martin Pilar, Serrano Manuel, Flores Juana Maria, Leitges Michael, Diaz-Meco María T, Moscat Jorge, ;2004;The EMBO journal;23;4595-605; 15526032
Regulation of mature T lymphocyte proliferation and differentiation by Par-4.;Lafuente María José, Martin Pilar, Garcia-Cao Isabel, Diaz-Meco María Teresa, Serrano Manuel, Moscat Jorge, ;2003;The EMBO journal;22;4689-98; 12970181

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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STOCK Pawrtm1Jmos/Cnbc