C57BL/6-Tg(PF4-RAPGEF1)6A6Cgu/Cnbc
| Status | Available to order |
| EMMA ID | EM:06334 |
| International strain name | C57BL/6-Tg(PF4-RAPGEF1)6A6Cgu/Cnbc |
| Alternative name | tgPF4-C3G |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(PF4-RAPGEF1)6A6Cgu, |
| Gene/Transgene symbol | Tg(PF4-RAPGEF1)6A6Cgu |
Information from provider
| Provider | Carmen Guerrero |
| Provider affiliation | Medicine, Center for Cancer Research (IBMCC)-University of Salamanca |
| Genetic information | Complete human RAPGEF1 (C3G) cDNA is expressed under the rat PF4 gene promoter that specifically directs the expression to megakaryocytes and platelets. The plasmid used to create the chimeras also contains loxP sequences and neo selection cassette. The transgenic mouse model was developed using genOway´s "Safe DNA transgenesis technology TM". This was achieved in three phases. First, the plasmid containing the transgene was transfected into ES cells by electroporation in order to generate stably transfected ES cell clones. These clones were then screened in vitro for transgene integrity and quantification using PCR and Southern blot. Finally, based on the screening results, 5 ES cell clones were selected and processed further for ES cell blastocyst injection. The transgene integration was a random event. |
| Phenotypic information | Mice expressing C3G transgene in platelets show shorter bleeding times and display higher platelet activity than wild-type siblings. Also, C3G transgenic mice show higher neutrophil counts than control mice. |
| Breeding history | Animals have been inbred for at least 15 generations |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J |
Literature references
- C3G transgenic mouse models with specific expression in platelets reveal a new role for C3G in platelet clotting through its GEF activity.;Gutiérrez-Herrero Sara, Maia Vera, Gutiérrez-Berzal Javier, Calzada Nuria, Sanz María, González-Manchón Consuelo, Pericacho Miguel, Ortiz-Rivero Sara, González-Porras José R, Arechederra María, Porras Almudena, Guerrero Carmen, ;2012;Biochimica et biophysica acta;1823;1366-77; 22659131