B6.129P2-Brca2tm2Mak/Cnbc

Status

Available to order

EMMA IDEM:06747
Citation informationRRID:IMSR_EM:06747 

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International strain nameB6.129P2-Brca2tm2Mak/Cnbc
Alternative nameBrCa2
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolBrca2tm2Mak
Gene/Transgene symbolBrca2

Information from provider

ProviderTak W Mak
Provider affiliationMak Lab, Campbell Family Breast Cancer reseach Institute
Genetic informationConditional targeted mutation of exons 9 and 10 flanked by loxP sites.
Phenotypic informationBrca2-null mice have a defect in embryonic cellular proliferation and die in utero. tBrca2/ T cells were more likely than wild-type cells to undergo spontaneous apoptosis, but apoptosed normally in response to restimulation or DNA-damaging stress signals.
Breeding historyBackcrossed 10 times to C57BL/6.
References
  • Loss of Brca2 and p53 synergistically promotes genomic instability and deregulation of T-cell apoptosis.;Cheung Alison M Y, Hande M Prakash, Jalali Farid, Tsao Ming-Sound, Skinnider Brian, Hirao Atsushi, McPherson J Peter, Karaskova Jana, Suzuki Akira, Wakeham Andrew, You-Ten Annick, Elia Andrew, Squire Jeremy, Bristow Rob, Hakem Razqallah, Mak Tak W, ;2002;Cancer research;62;6194-204; 12414647
  • Brca2 deficiency does not impair mammary epithelium development but promotes mammary adenocarcinoma formation in p53(+/-) mutant mice.;Cheung Alison M Y, Elia Andrew, Tsao Ming-Sound, Done Susan, Wagner Kay-Uwe, Hennighausen Lothar, Hakem Razqallah, Mak Tak W, ;2004;Cancer research;64;1959-65; 15026330
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivinghomozygous C57BL/6J males, wild-type C57BL/6J females
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (gene matching)
  • abnormal rib morphology / MGI
  • abnormal sternum morphology / MGI
  • decreased bone marrow cell number / MGI
  • kinked tail / MGI
  • abnormal branching of the mammary ductal tree / MGI
  • spleen hypoplasia / MGI
  • open neural tube / MGI
  • small ovary / MGI
  • small testis / MGI
  • abnormal epidermal layer morphology / MGI
  • abnormal epidermis stratum basale morphology / MGI
  • abnormal epidermis stratum spinosum morphology / MGI
  • absent epidermis stratum corneum / MGI
  • decreased body weight / MGI
  • impaired hematopoiesis / MGI
  • abnormal embryo development / MGI
  • decreased embryo size / MGI
  • abnormal visceral yolk sac morphology / MGI
  • male infertility / MGI
  • female infertility / MGI
  • neoplasm / MGI
  • increased tumor incidence / MGI
  • increased T cell derived lymphoma incidence / MGI
  • increased carcinoma incidence / MGI
  • decreased tumor incidence / MGI
  • abnormal skin morphology / MGI
  • abnormal motor capabilities/coordination/movement / MGI
  • premature death / MGI
  • abnormal developmental patterning / MGI
  • abnormal embryonic tissue morphology / MGI
  • abnormal extraembryonic tissue morphology / MGI
  • abnormal axial skeleton morphology / MGI
  • no abnormal phenotype detected / MGI
  • abnormal hematopoietic system morphology/development / MGI
  • absent ovarian follicles / MGI
  • abnormal chromosome morphology / MGI
  • embryonic growth retardation / MGI
  • increased mortality induced by ionizing radiation / MGI
  • abnormal mitosis / MGI
  • fetal growth retardation / MGI
  • decreased incidence of tumors by chemical induction / MGI
  • enlarged allantois / MGI
  • absent germ cells / MGI
  • increased thymus weight / MGI
  • abnormal amnion morphology / MGI
  • sepsis / MGI
  • azoospermia / MGI
  • growth/size/body region phenotype / MGI
  • reproductive system phenotype / MGI
  • abnormal cell physiology / MGI
  • increased apoptosis / MGI
  • early cellular replicative senescence / MGI
  • abnormal DNA repair / MGI
  • decreased survivor rate / MGI
  • decreased common myeloid progenitor cell number / MGI
  • chromosomal instability / MGI
  • increased sensitivity to induced cell death / MGI
  • adrenal gland hyperplasia / MGI
  • decreased fetal weight / MGI
  • decreased birth weight / MGI
  • increased mammary gland tumor incidence / MGI
  • decreased tumor latency / MGI
  • abnormal double-strand DNA break repair / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • neonatal lethality, incomplete penetrance / MGI
  • perinatal lethality, incomplete penetrance / MGI
  • prenatal lethality, complete penetrance / MGI
  • embryonic lethality, complete penetrance / MGI
  • embryonic lethality between somite formation and embryo turning, complete penetrance / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • prenatal lethality, incomplete penetrance / MGI
  • embryonic lethality during organogenesis, incomplete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • abnormal proamniotic cavity morphology / MGI
  • decreased fibroblast proliferation / MGI
  • increased lymphoma incidence / MGI
  • ovary degeneration / MGI

Literature references

  • Loss of Brca2 and p53 synergistically promotes genomic instability and deregulation of T-cell apoptosis.;Cheung Alison M Y, Hande M Prakash, Jalali Farid, Tsao Ming-Sound, Skinnider Brian, Hirao Atsushi, McPherson J Peter, Karaskova Jana, Suzuki Akira, Wakeham Andrew, You-Ten Annick, Elia Andrew, Squire Jeremy, Bristow Rob, Hakem Razqallah, Mak Tak W, ;2002;Cancer research;62;6194-204; 12414647
  • Brca2 deficiency does not impair mammary epithelium development but promotes mammary adenocarcinoma formation in p53(+/-) mutant mice.;Cheung Alison M Y, Elia Andrew, Tsao Ming-Sound, Done Susan, Wagner Kay-Uwe, Hennighausen Lothar, Hakem Razqallah, Mak Tak W, ;2004;Cancer research;64;1959-65; 15026330

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
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Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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