MGI phenotypes (allele matching)
- no phenotypic analysis / MGI
B6.129P2-Gja1tm9.1Kwi/Cnrm
| Status | Available to order |
| EMMA ID | EM:06788 |
| International strain name | B6.129P2-Gja1tm9.1Kwi/Cnrm |
| Alternative name | Cx43floxCx43kiECFP |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Gja1tm9.1Kwi, |
| Gene/Transgene symbol | Gja1 |
Information from provider
| Provider | Klaus Willecke |
| Provider affiliation | Molecular genetics, LIMES Institute, University of Bonn |
| Genetic information | The Cx43 (Gja1) coding region is flanked by loxP sites. After cre recombinase-mediated deletion the ECFP protein is expressed under control of the endogenous Cx43 promoter. |
| Phenotypic information | Hetero- and homozygous fertile. After cre recombinase-mediated deletion homozygous lethal at P0 due to a heart failure. |
| Breeding history | Targeted HM-1 cells were injected in C57BL/6NCrl blastocysts, chimeras were backcrossed to C57BL/6NCrl and offspring over more than 10 generations to C57BL/6JCrl. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Autosomal dominant palmoplantar keratoderma and congenital alopecia / Orphanet_1010
- Oculodentodigital dysplasia / Orphanet_2710
- Craniometaphyseal dysplasia / Orphanet_1522
- Syndactyly type 3 / Orphanet_93404
- Erythrokeratodermia variabilis / Orphanet_317
Literature references
- Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity.;Degen Joachim, Dublin Pavel, Zhang Jiong, Dobrowolski Radoslaw, Jokwitz Melanie, Karram Khalad, Trotter Jacqueline, Jabs Ronald, Willecke Klaus, Steinhäuser Christian, Theis Martin, ;2012;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;26;4576-83; 22859373