B6.Cg-Tg(Krt1-15-EGFP)2Cot/Cnbc
| Status | Available to order |
| EMMA ID | EM:06792 |
| Citation information | RRID:IMSR_EM:06792 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(Krt1-15-EGFP)2Cot/Cnbc |
| Alternative name | B6.Cg-Tg(Krt1-15-EGFP)2Cot/J |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Krt1-15-EGFP)2Cot |
| Gene/Transgene symbol | Tg(Krt1-15-EGFP)2Cot |
Information from provider
| Provider | Jesus Espada |
| Provider affiliation | Departamento de Biologia del Cancer, Instituto de Investigaciones Biomedicas "Alberto Sols" CSIC-UAM |
| Additional owner | Dr. George Cotsarelis, University of Pennsylvania, Dermatology Department, Philadelphia PA, USA |
| Genetic information | The transgene comprises the entire 5.0-kb DNA segment that separates the Krt1-19 and Krt1-15 genes in the mouse genome and that contains the Krt1-15 promoter region joined to the cDNA encoding enhanced green fluorescent protein (EGFP). |
| Phenotypic information | The Krt1-15 promoter directs expression to the bulge region of the hair follicle; fluorescence activated cell sorting (FACS ) analysis demonstrated that highest EGFP expression occurs in bulge cells showing high surface expression of the epithelial stem cell marker integrin beta 1 (fibronectin receptor beta). |
| Breeding history | A transgenic construct containing an Enhanced Green Fluorescent Protein (EGFP) gene under the control of the mouse keratin complex 1, acidic, gene 15 promoter was injected into fertilized B6SJLF1/J mouse eggs. Founder animals were bred to C57BL/6J mice and backcrossed to the same for a total of ten generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Literature references
- Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge.;Liu Yaping, Lyle Stephen, Yang Zaixin, Cotsarelis George, ;2003;The Journal of investigative dermatology;121;963-8; 14708593
- Capturing and profiling adult hair follicle stem cells.;Morris Rebecca J, Liu Yaping, Marles Lee, Yang Zaixin, Trempus Carol, Li Shulan, Lin Jamie S, Sawicki Janet A, Cotsarelis George, ;2004;Nature biotechnology;22;411-7; 15024388