B6.Cg-Tg(Krt1-15-EGFP)2Cot/Cnbc

Status	Available to order
EMMA ID	EM:06792
International strain name	B6.Cg-Tg(Krt1-15-EGFP)2Cot/Cnbc
Alternative name	B6.Cg-Tg(Krt1-15-EGFP)2Cot/J
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Krt1-15-EGFP)2Cot,
Gene/Transgene symbol	Tg(Krt1-15-EGFP)2Cot

Information from provider

Provider	Jesus Espada
Provider affiliation	Departamento de Biologia del Cancer, Instituto de Investigaciones Biomedicas "Alberto Sols" CSIC-UAM
Additional owner	Dr. George Cotsarelis, University of Pennsylvania, Dermatology Department, Philadelphia PA, USA
Genetic information	The transgene comprises the entire 5.0-kb DNA segment that separates the Krt1-19 and Krt1-15 genes in the mouse genome and that contains the Krt1-15 promoter region joined to the cDNA encoding enhanced green fluorescent protein (EGFP).
Phenotypic information	The Krt1-15 promoter directs expression to the bulge region of the hair follicle; fluorescence activated cell sorting (FACS ) analysis demonstrated that highest EGFP expression occurs in bulge cells showing high surface expression of the epithelial stem cell marker integrin beta 1 (fibronectin receptor beta).
Breeding history	A transgenic construct containing an Enhanced Green Fluorescent Protein (EGFP) gene under the control of the mouse keratin complex 1, acidic, gene 15 promoter was injected into fertilized B6SJLF1/J mouse eggs. Founder animals were bred to C57BL/6J mice and backcrossed to the same for a total of ten generations.
References	Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge.;Liu Yaping, Lyle Stephen, Yang Zaixin, Cotsarelis George, ;2003;The Journal of investigative dermatology;121;963-8; 14708593 Capturing and profiling adult hair follicle stem cells.;Morris Rebecca J, Liu Yaping, Marles Lee, Yang Zaixin, Trempus Carol, Li Shulan, Lin Jamie S, Sawicki Janet A, Cotsarelis George, ;2004;Nature biotechnology;22;411-7; 15024388
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Literature references

Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge.;Liu Yaping, Lyle Stephen, Yang Zaixin, Cotsarelis George, ;2003;The Journal of investigative dermatology;121;963-8; 14708593
Capturing and profiling adult hair follicle stem cells.;Morris Rebecca J, Liu Yaping, Marles Lee, Yang Zaixin, Trempus Carol, Li Shulan, Lin Jamie S, Sawicki Janet A, Cotsarelis George, ;2004;Nature biotechnology;22;411-7; 15024388

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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