C57BL/6N-A^tm1Brd 4933434E20Rik^{tm1a(EUCOMM)Wtsi}/WtsiCnrm

Status	Available to order
EMMA ID	EM:06846
Citation information	RRID:IMSR_EM:06846 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-A^tm1Brd 4933434E20Rik^{tm1a(EUCOMM)Wtsi}/WtsiCnrm
Alternative name	EPD0371_1_D02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	4933434E20Rik^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	4933434E20Rik
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0371_1_D02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased circulating HDL cholesterol level / IMPC
increased circulating alanine transaminase level / IMPC
decreased circulating cholesterol level / IMPC
decreased circulating serum albumin level / IMPC
decreased circulating fructosamine level / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

decreased circulating HDL cholesterol level / IMPC
preweaning lethality, complete penetrance / IMPC
decreased circulating cholesterol level / IMPC
decreased circulating fructosamine level / IMPC
increased circulating alanine transaminase level / IMPC
decreased circulating serum albumin level / IMPC

MGI phenotypes (allele matching)

abnormal vertebral body morphology / MGI
overriding aortic valve / MGI
abnormal interatrial septum morphology / MGI
abnormal atrioventricular cushion morphology / MGI
enlarged liver sinusoidal spaces / MGI
abnormal pineal gland morphology / MGI
abnormal forebrain morphology / MGI
abnormal cerebral cortex morphology / MGI
abnormal hindbrain morphology / MGI
fused dorsal root ganglion / MGI
small superior cervical ganglion / MGI
abnormal lens morphology / MGI
abnormal larynx morphology / MGI
abnormal heart atrium morphology / MGI
anal atresia / MGI
intestinal/bowel diverticulum / MGI
urinary bladder hypoplasia / MGI
abnormal eye muscle morphology / MGI
abnormal Mullerian duct morphology / MGI
abnormal heart right atrium morphology / MGI
right aortic arch / MGI
retroesophageal right subclavian artery / MGI
abnormal vertebral arch morphology / MGI
absent vertebral arch / MGI
fusion of vertebral arches / MGI
abnormal heart position or orientation / MGI
arteriovenous malformation / MGI
dorsal root ganglion hypoplasia / MGI
abnormal brain internal capsule morphology / MGI
enlarged lymphatic vessel / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
abnormal inferior vena cava valve morphology / MGI
abnormal pectinate muscle morphology / MGI
abnormal aortic valve cusp morphology / MGI
abnormal pulmonary valve cusp morphology / MGI
herniated liver / MGI
double ureter / MGI
absent hypoglossal canal / MGI
thin hypoglossal nerve / MGI
absent hypoglossal nerve / MGI
abnormal hypoglossal nerve topology / MGI
absent segment of posterior cerebral artery / MGI
subcutaneous edema / MGI
abnormal vitelline vein connection / MGI
absent ductus venosus valve / MGI
abnormal ductus venosus valve morphology / MGI
abnormal ductus venosus valve topology / MGI
absent costovertebral joint / MGI
abnormal dural venous sinus morphology / MGI
absent lobe of thyroid gland / MGI
retro-esophageal left subclavian artery / MGI
abnormal infrahyoid muscle connection / MGI
reduced sympathetic cervical ganglion size / MGI
blood in lymph vessels / MGI
abnormal vertebral artery topology / MGI
abnormal Wolffian duct connection / MGI
embryo cyst / MGI
heterochrony / MGI

MGI phenotypes (gene matching)

abnormal vertebral body morphology / MGI
overriding aortic valve / MGI
abnormal interatrial septum morphology / MGI
abnormal atrioventricular cushion morphology / MGI
enlarged liver sinusoidal spaces / MGI
abnormal pineal gland morphology / MGI
abnormal forebrain morphology / MGI
abnormal cerebral cortex morphology / MGI
abnormal hindbrain morphology / MGI
fused dorsal root ganglion / MGI
small superior cervical ganglion / MGI
abnormal lens morphology / MGI
abnormal larynx morphology / MGI
abnormal heart atrium morphology / MGI
anal atresia / MGI
intestinal/bowel diverticulum / MGI
urinary bladder hypoplasia / MGI
abnormal eye muscle morphology / MGI
abnormal Mullerian duct morphology / MGI
abnormal heart right atrium morphology / MGI
right aortic arch / MGI
retroesophageal right subclavian artery / MGI
abnormal vertebral arch morphology / MGI
absent vertebral arch / MGI
fusion of vertebral arches / MGI
abnormal heart position or orientation / MGI
arteriovenous malformation / MGI
dorsal root ganglion hypoplasia / MGI
abnormal brain internal capsule morphology / MGI
enlarged lymphatic vessel / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
abnormal inferior vena cava valve morphology / MGI
abnormal pectinate muscle morphology / MGI
abnormal aortic valve cusp morphology / MGI
abnormal pulmonary valve cusp morphology / MGI
herniated liver / MGI
double ureter / MGI
absent hypoglossal canal / MGI
thin hypoglossal nerve / MGI
absent hypoglossal nerve / MGI
abnormal hypoglossal nerve topology / MGI
absent segment of posterior cerebral artery / MGI
subcutaneous edema / MGI
abnormal vitelline vein connection / MGI
absent ductus venosus valve / MGI
abnormal ductus venosus valve morphology / MGI
abnormal ductus venosus valve topology / MGI
absent costovertebral joint / MGI
abnormal dural venous sinus morphology / MGI
absent lobe of thyroid gland / MGI
retro-esophageal left subclavian artery / MGI
abnormal infrahyoid muscle connection / MGI
reduced sympathetic cervical ganglion size / MGI
blood in lymph vessels / MGI
abnormal vertebral artery topology / MGI
abnormal Wolffian duct connection / MGI
embryo cyst / MGI
heterochrony / MGI
persistent trigeminal artery / MGI

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd 4933434E20Riktm1a(EUCOMM)Wtsi/WtsiCnrm