C57BL/6-Tg(UBC-GFP,-Fkbp11)HPsou/Orl
| Status | Available to order |
| EMMA ID | EM:06914 |
| Citation information | RRID:IMSR_EM:06914 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Tg(UBC-GFP,-Fkbp11)HPsou/Orl |
| Alternative name | FKBPhigh |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(UBC-GFP,-Fkbp11)HPsou |
| Gene/Transgene symbol | Tg(UBC-GFP,-Fkbp11)HPsou |
Information from provider
| Provider | Pauline Soulas-Sprauel |
| Provider affiliation | CRBS (Centre de Recherche en Biomédecine), Inserm UMR 1109 (Immuno-Rhumatologie Moléculaire, IRM) |
| Genetic information | This FKBP11high lentiviral transgenic mouse line carries one copy of stable lentiviral integration with GFP reporter. GFP and FKBP19 (protein encoded by Fkbp11 gene) are coexpressed in a stoichiometric manner thanks to a sequence encoding a T2A peptide (derived from picornavirus) between GFP and Fkbp11 sequences. |
| Phenotypic information | FKBP11 mRNA is 7 times over expressed in bone marrow, 36 times in lymph nodes, 8 times in spleen, and 6 times in purified splenic B cells. Normal development. Splenic hyperplasia concerning all lymphoid subpopulations, especially B cells. Increased B cell response to T-dependent antigen in vivo. Increased production of autoantibodies (IgG antidsDNA for example). IgG3 hypergammaglobulinemia. Increased plasma cell differentiation. We have referenced a FKBP11 LOW lentigenic mouse line in EMMA program, with a smaller over expression of FKBP11: the phenotype of this LOW line is very light compared to this "high" line: no hypergammaglobulinemia, no splenic hyperplasia and no autoantibody production in the LOW line. |
| Breeding history | The lentiviral transgenic strain was produced on C57BL/6 background and maintained in the same background by backcrosses. We have selected a one-copy founder which has been studied on different generations and the line is stable (good Mendelian transmission and reproducible results on several generations). |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J males |
Literature references
- Overexpression of Fkbp11, a feature of lupus B cells, leads to B cell tolerance breakdown and initiates plasma cell differentiation.;Ruer-Laventie Julie, Simoni Léa, Schickel Jean-Nicolas, Soley Anne, Duval Monique, Knapp Anne-Marie, Marcellin Luc, Lamon Delphine, Korganow Anne-Sophie, Martin Thierry, Pasquali Jean-Louis, Soulas-Sprauel Pauline, ;2015;Immunity, inflammation and disease;3;265-79; 26417441