MGI phenotypes (allele matching)
B6N.Cg-Piwil2tm2.2Doca/Cnrm
| Status | Available to order |
| EMMA ID | EM:07098 |
| International strain name | B6N.Cg-Piwil2tm2.2Doca/Cnrm |
| Alternative name | MiliNull |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Piwil2tm2.2Doca, |
| Gene/Transgene symbol | Piwil2 |
Information from provider
| Provider | Donal O'Carroll |
| Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
| Genetic information | Exon 21 was flanked by two loxP sites for the formation of a null allele through Cre recombination. |
| Phenotypic information | Male sterility due to a meiotic arrest, transposons derepression due to DNA-methylation defect. |
| Breeding history | The mouse was backcrossed 8 times with C57BL/6N mice. |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- Multiple epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis.;Di Giacomo Monica, Comazzetto Stefano, Saini Harpreet, De Fazio Serena, Carrieri Claudia, Morgan Marcos, Vasiliauskaite Lina, Benes Vladimir, Enright Anton J, O'Carroll Dónal, ;2013;Molecular cell;50;601-8; 23706823
- G9a co-suppresses LINE1 elements in spermatogonia.;Di Giacomo Monica, Comazzetto Stefano, Sampath Srihari C, Sampath Srinath C, O'Carroll Dónal, ;2014;Epigenetics & chromatin;7;24; 25276231