C57BL/6NTac-Ncf2tm1a(EUCOMM)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:07176
Citation informationRRID:IMSR_EM:07176 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6NTac-Ncf2tm1a(EUCOMM)Wtsi/WtsiH
Alternative nameEPD0240_5_B03
Strain typeTargeted Mutant Strains
Allele/Transgene symbolNcf2tm1a(EUCOMM)Wtsi
Gene/Transgene symbolNcf2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0240_5_B03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • decreased leukocyte cell number / IMPC
  • increased mean platelet volume / IMPC
  • decreased regulatory T cell number / IMPC
  • decreased T cell number / IMPC
  • decreased circulating thyroxine level / IMPC
  • decreased NK T cell number / IMPC
  • decreased NK cell number / IMPC
  • increased circulating iron level / IMPC
  • decreased grip strength / IMPC
  • decreased alpha-beta T cell number / IMPC
  • increased KLRG1-positive NK cell number / IMPC
  • obsolete decreased CD4-positive, alpha beta T cell number / IMPC
  • obsolete decreased CD8-positive, alpha beta T cell number / IMPC
IMPC phenotypes (gene matching)
  • decreased regulatory T cell number / IMPC
  • increased KLRG1-positive NK cell number / IMPC
  • increased mean platelet volume / IMPC
  • decreased T cell number / IMPC
  • decreased alpha-beta T cell number / IMPC
  • decreased grip strength / IMPC
  • decreased NK T cell number / IMPC
  • obsolete decreased CD4-positive, alpha beta T cell number / IMPC
  • increased circulating iron level / IMPC
  • decreased circulating thyroxine level / IMPC
  • obsolete decreased CD8-positive, alpha beta T cell number / IMPC
  • decreased NK cell number / IMPC
  • decreased leukocyte cell number / IMPC
MGI phenotypes (gene matching)
  • increased activated T cell number / MGI
  • lung inflammation / MGI
  • premature death / MGI
  • abnormal spleen germinal center morphology / MGI
  • increased urine protein level / MGI
  • abnormal redox activity / MGI
  • increased anti-double stranded DNA antibody level / MGI
  • increased susceptibility to systemic lupus erythematosus / MGI
  • increased B cell number / MGI
  • increased T cell number / MGI
  • abnormal renal glomerulus morphology / MGI
  • increased susceptibility to fungal infection / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • increased follicular B cell number / MGI
  • increased germinal center B cell number / MGI
  • increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / MGI
  • increased CD4-positive, alpha-beta memory T cell number / MGI
  • abnormal mesangial matrix morphology / MGI
  • abnormal kidney interstitium morphology / MGI
  • glomerular crescent / MGI
  • renal glomerular protein deposits / MGI
  • renal glomerular immunoglobulin deposits / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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Practical information

Genotyping protocol

Example health report
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