B6.Cg-Cdkl5tm1.1Cogr/Cnrm
| Status | Available to order |
| EMMA ID | EM:07213 |
| International strain name | B6.Cg-Cdkl5tm1.1Cogr/Cnrm |
| Alternative name | Cdkl5cKO B6N |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Cdkl5tm1.1Cogr, |
| Gene/Transgene symbol | Cdkl5 |
Information from provider
| Provider | Cornelius Gross |
| Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
| Additional owner | Dr. Elena Amendola, EMBL Mouse Molecular Unit, Monterotondo, Italy |
| Genetic information | Cdkl5 exon4 was targeted by homologous recombination. Exon 4 was flanked by two loxP sites followed by neomycin cassette flanked by Flp sites used for removal of the cassette. |
| Phenotypic information | Heterozygous and homozygous conditional Cdkl5 mice do not show any Rett-like phenotype. The conditional CDKL5 mice could be considered like wild-type mice. Upon crossing these mice with a different Cre strain it is possible to generate mice with a Cdkl5 specific inactivation. |
| Breeding history | Mutant mice were crossed with C57BL/6J FLP-deleter mice (Farley et al., 2000) made congenic in B6N genetic background (N>10). After that mice were bred for 7 generations in a C57BL/6N genetic background. The first mating (FLP) was not considered in the calculation of the generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- CDKL5-related epileptic encephalopathy / Orphanet_505652
- Early infantile epileptic encephalopathy / Orphanet_1934
- Atypical Rett syndrome / Orphanet_3095
- West syndrome / Orphanet_3451
Literature references
- Mapping pathological phenotypes in a mouse model of CDKL5 disorder.;Amendola Elena, Zhan Yang, Mattucci Camilla, Castroflorio Enrico, Calcagno Eleonora, Fuchs Claudia, Lonetti Giuseppina, Silingardi Davide, Vyssotski Alexei L, Farley Dominika, Ciani Elisabetta, Pizzorusso Tommaso, Giustetto Maurizio, Gross Cornelius T, ;2014;PloS one;9;e91613; 24838000