B6.129P2-Hipk1tm1Mak/Cnbc

Status

Available to order

EMMA IDEM:07222
International strain nameB6.129P2-Hipk1tm1Mak/Cnbc
Alternative nameP53bk / HIPK1 (Hipk1)
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolHipk1tm1Mak,
Gene/Transgene symbolHipk1

Information from provider

ProviderTak W Mak
Provider affiliationDivision of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute
Genetic informationTargeting vector replaced 2.7 kb of genomic DNA containing the first exon of Hipk1 (encoding the putative kinase domain) with a neomycin-resistance cassette inserted in sense orientation to Hipk1 transcription.
Phenotypic informationGene-targeted Hipk1 -/- mice were grossly normal but oncogenically transformed; Hipk1 -/- mouse embryonic fibroblasts exhibited reduced transcription of Mdm2 and were more susceptible than transformed Hipk1 +/+ cells to apoptosis induced by DNA damage. Carcinogen-treated Hipk1 -/- mice developed fewer and smaller skin tumors than Hipk1 +/+ mice.
Breeding historyPure C57BL/6, backcrossed 10 times.
References
  • Characterization of cells and gene-targeted mice deficient for the p53-binding kinase homeodomain-interacting protein kinase 1 (HIPK1).;Kondo Seiji, Lu Ying, Debbas Michael, Lin Athena W, Sarosi Ildiko, Itie Annick, Wakeham Andrew, Tuan JoAnn, Saris Chris, Elliott Gary, Ma Weili, Benchimol Samuel, Lowe Scott W, Mak Tak Wah, Thukral Sushil K, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;5431-6; 12702766
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivinghomozygous C57BL/6J

Disease and phenotype information

MGI phenotypes (allele matching)
  • decreased incidence of tumors by chemical induction / MGI

Literature references

  • Characterization of cells and gene-targeted mice deficient for the p53-binding kinase homeodomain-interacting protein kinase 1 (HIPK1).;Kondo Seiji, Lu Ying, Debbas Michael, Lin Athena W, Sarosi Ildiko, Itie Annick, Wakeham Andrew, Tuan JoAnn, Saris Chris, Elliott Gary, Ma Weili, Benchimol Samuel, Lowe Scott W, Mak Tak Wah, Thukral Sushil K, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;5431-6; 12702766

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).