B6;129P2-Cycstm1Mak/Cnbc

Status

Available to order

EMMA IDEM:07227
International strain nameB6;129P2-Cycstm1Mak/Cnbc
Alternative nameCytochrome C 129Ola/B6
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolCycstm1Mak,
Gene/Transgene symbolCycs

Information from provider

ProviderTak W Mak
Provider affiliationDivision of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute
Genetic informationWe used site-directed mutagenesis to mutate the codon AAA that starts at nucleotide 216. The K72A (KA) knock-in allele was introduced into the murine genomic cytochrome c (Cycs) locus in embryonic stem cells by homologous recombination.
Phenotypic informationWe generated knock-in mice expressing a mutant cytochrome c (Cycs gene, KA allele), which retains normal electron transfer function but fails to activate Apaf1. Most KA/KA mice displayed embryonic or perinatal lethality caused by defects in the central nervous system, and surviving mice exhibited impaired lymphocyte homeostasis.
References
  • Specific ablation of the apoptotic functions of cytochrome C reveals a differential requirement for cytochrome C and Apaf-1 in apoptosis.;Hao Zhenyue, Duncan Gordon S, Chang Chia-Che, Elia Andrew, Fang Min, Wakeham Andrew, Okada Hitoshi, Calzascia Thomas, Jang YingJu, You-Ten Annick, Yeh Wen-Chen, Ohashi Pamela, Wang Xiaodong, Mak Tak W, ;2005;Cell;121;579-591; 15907471
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6J

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • decreased leukocyte cell number / MGI
  • absent pre-B cells / MGI
  • abnormal pituitary gland morphology / MGI
  • spleen hyperplasia / MGI
  • weakness / MGI
  • hindlimb paralysis / MGI
  • forebrain hypoplasia / MGI
  • abnormal cerebral cortex morphology / MGI
  • abnormal thalamus morphology / MGI
  • abnormal hypothalamus morphology / MGI
  • midbrain hyperplasia / MGI
  • exencephaly / MGI
  • cranioschisis / MGI
  • bidirectional circling / MGI
  • impaired limb coordination / MGI
  • thymus hypoplasia / MGI
  • hydroencephaly / MGI
  • intracerebral hemorrhage / MGI
  • premature death / MGI
  • decreased cellular sensitivity to gamma-irradiation / MGI
  • thymus atrophy / MGI
  • increased spleen weight / MGI
  • decreased B cell number / MGI
  • decreased T cell number / MGI
  • cachexia / MGI
  • perinatal lethality, incomplete penetrance / MGI
  • prenatal lethality, incomplete penetrance / MGI
  • prominent forehead / MGI

Literature references

  • Specific ablation of the apoptotic functions of cytochrome C reveals a differential requirement for cytochrome C and Apaf-1 in apoptosis.;Hao Zhenyue, Duncan Gordon S, Chang Chia-Che, Elia Andrew, Fang Min, Wakeham Andrew, Okada Hitoshi, Calzascia Thomas, Jang YingJu, You-Ten Annick, Yeh Wen-Chen, Ohashi Pamela, Wang Xiaodong, Mak Tak W, ;2005;Cell;121;579-591; 15907471

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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