B6.129P2-Diablo^tm1Mak/Cnbc

Status	Available to order
EMMA ID	EM:07235
International strain name	B6.129P2-Diablo^tm1Mak/Cnbc
Alternative name	SMAC (Diablo)
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Diablo^tm1Mak,
Gene/Transgene symbol	Diablo

Information from provider

Provider	Tak W Mak
Provider affiliation	Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute
Genetic information	Exons 2 to 4 were replaced with PGK-neo and diphtheria toxin A (DT-A) was added for negative selection.
Phenotypic information	Diablo (Smac) -/- mice were viable, grew, and matured normally and did not show any histological abnormalities. Although the cleavage in vitro of procaspase-3 was inhibited in lysates of Smac -/- cells, all types of cultured Smac -/- cells tested responded normally to all apoptotic stimuli applied. There were also no detectable differences in Fas-mediated apoptosis in the liver in vivo. Our data strongly suggest the existence of a redundant molecule or molecules capable of compensating for a loss of Smac function.
Breeding history	Backcrossed 10 times to C57BL/6.
References	Requirement for Casper (c-FLIP) in regulation of death receptor-induced apoptosis and embryonic development.;Yeh W C, Itie A, Elia A J, Ng M, Shu H B, Wakeham A, Mirtsos C, Suzuki N, Bonnard M, Goeddel D V, Mak T W, ;2000;Immunity;12;633-42; 10894163 Generation and characterization of Smac/DIABLO-deficient mice.;Okada Hitoshi, Suh Woong-Kyung, Jin Jianping, Woo Minna, Du Chunying, Elia Andrew, Duncan Gordon S, Wakeham Andrew, Itie Annick, Lowe Scott W, Wang Xiaodong, Mak Tak W, ;2002;Molecular and cellular biology;22;3509-17; 11971981
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6J

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal dominant non-syndromic sensorineural deafness type DFNA / Orphanet_90635

Literature references

Requirement for Casper (c-FLIP) in regulation of death receptor-induced apoptosis and embryonic development.;Yeh W C, Itie A, Elia A J, Ng M, Shu H B, Wakeham A, Mirtsos C, Suzuki N, Bonnard M, Goeddel D V, Mak T W, ;2000;Immunity;12;633-42; 10894163
Generation and characterization of Smac/DIABLO-deficient mice.;Okada Hitoshi, Suh Woong-Kyung, Jin Jianping, Woo Minna, Du Chunying, Elia Andrew, Duncan Gordon S, Wakeham Andrew, Itie Annick, Lowe Scott W, Wang Xiaodong, Mak Tak W, ;2002;Molecular and cellular biology;22;3509-17; 11971981

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6.129P2-Diablotm1Mak/Cnbc