B6.129P2-Pecam1tm1Mak/Cnbc

Status

Available to order

EMMA IDEM:07243
International strain nameB6.129P2-Pecam1tm1Mak/Cnbc
Alternative nameCD31/Pecam1
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolPecam1tm1Mak,
Gene/Transgene symbolPecam1

Information from provider

ProviderTak W Mak
Provider affiliationDivision of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute
Genetic informationA targeting vector was designed to disrupt exon 7 by inserting a pGK1neo resistance expression cassette in reverse orientation of CD31 transcription.
Phenotypic informationPecam1 (CD31)-deficient mice (CD31KO) are viable and born at the expected Mendelian frequency, remain healthy, and exhibit no obvious vascular developmental defects. In response to inflammatory challenge, polymorphonuclear leukocytes of CD31KO mice are arrested between the vascular endothelium and the basement membrane of inflammatory site mesenteric microvessels, confirming a role for CD31 in the migration of neutrophils through the subendothelial extracellular matrix.
Breeding historyBackcrossed 10 times to C57BL/6.
References
  • Generation and characterization of the Anp32e-deficient mouse.;Reilly Patrick T, Afzal Samia, Wakeham Andrew, Haight Jillian, You-Ten Annick, Zaugg Kathrin, Dembowy Joanna, Young Ashley, Mak Tak W, ;2010;PloS one;5;e13597; 21049064
  • Genetic evidence for functional redundancy of Platelet/Endothelial cell adhesion molecule-1 (PECAM-1): CD31-deficient mice reveal PECAM-1-dependent and PECAM-1-independent functions.;Duncan G S, Andrew D P, Takimoto H, Kaufman S A, Yoshida H, Spellberg J, de la Pompa J L, Elia A, Wakeham A, Karan-Tamir B, Muller W A, Senaldi G, Zukowski M M, Mak T W, ;1999;Journal of immunology (Baltimore, Md. : 1950);162;3022-30; 10072554
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6J

Disease and phenotype information

MGI phenotypes (allele matching)
  • increased susceptibility to induced arthritis / MGI
  • abnormal leukocyte physiology / MGI
  • joint inflammation / MGI
  • increased interferon-gamma secretion / MGI
  • kidney vascular congestion / MGI
  • liver inflammation / MGI
  • abnormal cytokine secretion / MGI
  • increased vascular permeability / MGI
  • pulmonary edema / MGI
  • pulmonary vascular congestion / MGI
  • liver vascular congestion / MGI
  • spleen vascular congestion / MGI
  • glomerular capillary congestion / MGI
  • abnormal lung morphology / MGI
  • impaired lung alveolus development / MGI
  • abnormal pulmonary alveolar sac morphology / MGI
  • abnormal pulmonary alveolus wall morphology / MGI
  • decreased lung endothelial cell migration / MGI

Literature references

  • Generation and characterization of the Anp32e-deficient mouse.;Reilly Patrick T, Afzal Samia, Wakeham Andrew, Haight Jillian, You-Ten Annick, Zaugg Kathrin, Dembowy Joanna, Young Ashley, Mak Tak W, ;2010;PloS one;5;e13597; 21049064
  • Genetic evidence for functional redundancy of Platelet/Endothelial cell adhesion molecule-1 (PECAM-1): CD31-deficient mice reveal PECAM-1-dependent and PECAM-1-independent functions.;Duncan G S, Andrew D P, Takimoto H, Kaufman S A, Yoshida H, Spellberg J, de la Pompa J L, Elia A, Wakeham A, Karan-Tamir B, Muller W A, Senaldi G, Zukowski M M, Mak T W, ;1999;Journal of immunology (Baltimore, Md. : 1950);162;3022-30; 10072554

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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