C.129(B6)-Foxe3tm1Pca/Ieg

Status

Available to order

EMMA IDEM:07336
International strain nameC.129(B6)-Foxe3tm1Pca/Ieg
Alternative nameFoxE3-ko
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolFoxe3tm1Pca,
Gene/Transgene symbolFoxe3

Information from provider

ProviderPeter Carlsson
Provider affiliationDept of Chemistry and Molecular Biology, University of Gothenburg
Genetic informationThe forkhead box of Foxe3 (from NcoI to NotI) was replaced by a lacZ-PGK-neo resistance cassette. The construct was linearized at the end of the long arm and electroporated into R1 ES cells. Targeted cell clones were used to generate chimeras through injection into C57BL/6 blastocysts. A knock-out line was established following germline transmission of targeted ES cells and made congenic with the Foxe3 strain (Sanyal and Hawkins, 1979) by crossing with BALB/c for five generations.

Disclaimer - Special restrictions:

1) Mutant development was phenotype driven. Although for some mutant lines, linkage data and the molecular characterization of the causative mutation has been published, EMMA has not genotyped the strains and can not provide genotyping protocols.
2) Some lines showed low penetrance of the phenotype.
3) Only sperm available. Rederivation service can not be offered.
4) EMMA quality control standards may not apply for these strains.
5) EMMA has not verified the breeding performance and the genetic background of the strains.

The genotype/phenotype of the mice is not confirmed by INFRAFRONTIER/EMMA. We strongly recommend that the recipient confirms genotype/phenotype upon receipt.
Phenotypic informationHomozygotes for the null mutation display microphthalmia, fusion of the lens and cornea, other corneal and lens abnormalities, and may fail to open their eyes.
Breeding historyBrother x sister
References
  • Foxe3 is required for morphogenesis and differentiation of the anterior segment of the eye and is sensitive to Pax6 gene dosage.;Blixt Asa, Landgren Henrik, Johansson Bengt R, Carlsson Peter, ;2007;Developmental biology;302;218-29; 17064680
  • Persistent FoxE3 expression blocks cytoskeletal remodeling and organelle degradation during lens fiber differentiation.;Landgren Henrik, Blixt Asa, Carlsson Peter, ;2008;Investigative ophthalmology & visual science;49;4269-77; 18539941
  • Pitx3 directly regulates Foxe3 during early lens development.;Ahmad Nafees, Aslam Muhammad, Muenster Doris, Horsch Marion, Khan Muhammad A, Carlsson Peter, Beckers Johannes, Graw Jochen, ;2013;The International journal of developmental biology;57;741-51; 24307298
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous BALB/c

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • cataract / MGI
  • small lens / MGI
  • fused cornea and lens / MGI
  • abnormal lens development / MGI
  • abnormal iris morphology / MGI
  • abnormal anterior eye segment morphology / MGI
  • abnormal corneal stroma morphology / MGI
  • abnormal corneal endothelium morphology / MGI
  • iris hypoplasia / MGI
  • anterior iris synechia / MGI
  • abnormal cornea morphology / MGI
  • abnormal iridocorneal angle / MGI
  • absent trabecular meshwork / MGI
  • abnormal ciliary body morphology / MGI
  • abnormal canal of Schlemm morphology / MGI
  • corneal vascularization / MGI

Literature references

  • Foxe3 is required for morphogenesis and differentiation of the anterior segment of the eye and is sensitive to Pax6 gene dosage.;Blixt Asa, Landgren Henrik, Johansson Bengt R, Carlsson Peter, ;2007;Developmental biology;302;218-29; 17064680
  • Persistent FoxE3 expression blocks cytoskeletal remodeling and organelle degradation during lens fiber differentiation.;Landgren Henrik, Blixt Asa, Carlsson Peter, ;2008;Investigative ophthalmology & visual science;49;4269-77; 18539941
  • Pitx3 directly regulates Foxe3 during early lens development.;Ahmad Nafees, Aslam Muhammad, Muenster Doris, Horsch Marion, Khan Muhammad A, Carlsson Peter, Beckers Johannes, Graw Jochen, ;2013;The International journal of developmental biology;57;741-51; 24307298

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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