C57BL/6NTac-Xbp1^{tm1a(EUCOMM)Wtsi}/IcsOrl

Status	Available to order
EMMA ID	EM:07516
Citation information	RRID:IMSR_EM:07516 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6NTac-Xbp1^{tm1a(EUCOMM)Wtsi}/IcsOrl
Alternative name	EPD0038_2_E10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Xbp1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Xbp1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	This mouse line originates from EUCOMM ES clone EPD0038_2_E10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6NTac males, wild-type C57BL/6NTac females
Stage of embryos	8-cell

Disease and phenotype information

IMPC phenotypes (allele matching)

increased body weight / IMPC
abnormal lens morphology / IMPC
cataract / IMPC
abnormal urination / IMPC
decreased circulating triglyceride level / IMPC
abnormal bone mineralization / IMPC
thrombocytopenia / IMPC
increased lean body mass / IMPC
abnormal vertebral arch morphology / IMPC
vertebral fusion / IMPC
abnormal behavior / IMPC
decreased T cell number / IMPC
decreased circulating cholesterol level / IMPC
increased total body fat amount / IMPC
increased grip strength / IMPC
increased bone mineral content / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

decreased circulating triglyceride level / IMPC
abnormal behavior / IMPC
decreased T cell number / IMPC
thrombocytopenia / IMPC
increased grip strength / IMPC
increased mean corpuscular volume / IMPC
preweaning lethality, complete penetrance / IMPC
increased total body fat amount / IMPC
increased mean corpuscular hemoglobin / IMPC
persistence of hyaloid vascular system / IMPC
decreased leukocyte cell number / IMPC
cataract / IMPC
decreased large unstained cell number / IMPC
decreased red blood cell distribution width / IMPC
decreased circulating cholesterol level / IMPC
increased lean body mass / IMPC
abnormal lens morphology / IMPC
vertebral fusion / IMPC
decreased lymphocyte cell number / IMPC
increased bone mineral content / IMPC
abnormal bone mineralization / IMPC
increased body weight / IMPC
abnormal vertebral arch morphology / IMPC
abnormal urination / IMPC

MGI phenotypes (gene matching)

abnormal heart development / MGI
thin ventricular wall / MGI
enlarged pericardium / MGI
absent trabeculae carneae / MGI
decreased cell proliferation / MGI
abnormal liver development / MGI
liver hypoplasia / MGI
small liver / MGI
wavy neural tube / MGI
anemia / MGI
impaired hematopoiesis / MGI
abnormal blood vessel morphology / MGI
decreased embryo size / MGI
anoxia / MGI
pallor / MGI
increased hepatocyte apoptosis / MGI
embryonic growth retardation / MGI
decreased hepatocyte proliferation / MGI
abnormal extraembryonic tissue physiology / MGI
myocardial necrosis / MGI
decreased embryo weight / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
embryo tissue necrosis / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

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C57BL/6NTac-Xbp1tm1a(EUCOMM)Wtsi/IcsOrl