C57BL/6NTac-Grhl3^{tm1a(EUCOMM)Wtsi}/IcsOrl

Status	Available to order
EMMA ID	EM:07681
Citation information	RRID:IMSR_EM:07681 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6NTac-Grhl3^{tm1a(EUCOMM)Wtsi}/IcsOrl
Alternative name	EPD0039_3_C02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Grhl3^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Grhl3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	This mouse line originates from EUCOMM ES clone EPD0039_3_C02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6NTac males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Van der Woude syndrome / Orphanet_888

IMPC phenotypes (allele matching)

increased circulating calcium level / IMPC
abnormal retina morphology / IMPC
increased startle reflex / IMPC
increased circulating phosphate level / IMPC
abnormal eye morphology / IMPC
decreased circulating triglyceride level / IMPC
decreased circulating alkaline phosphatase level / IMPC
abnormal locomotor activation / IMPC
increased circulating glucose level / IMPC
decreased blood urea nitrogen level / IMPC
increased circulating amylase level / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

increased startle reflex / IMPC
increased circulating phosphate level / IMPC
increased circulating glucose level / IMPC
decreased circulating alkaline phosphatase level / IMPC
decreased blood urea nitrogen level / IMPC
abnormal eye morphology / IMPC
preweaning lethality, complete penetrance / IMPC
abnormal retina morphology / IMPC
increased circulating amylase level / IMPC
increased circulating calcium level / IMPC
decreased circulating triglyceride level / IMPC
abnormal locomotor activation / IMPC

MGI phenotypes (gene matching)

cleft palate / MGI
abnormal vertebrae morphology / MGI
kyphosis / MGI
gastrointestinal hemorrhage / MGI
abnormal stomach epithelium morphology / MGI
abnormal intestine morphology / MGI
kinked tail / MGI
abnormal brain development / MGI
exencephaly / MGI
open neural tube / MGI
scaly skin / MGI
skin lesions / MGI
abnormal epidermal layer morphology / MGI
thick epidermis / MGI
epidermal hyperplasia / MGI
abnormal epidermis stratum basale morphology / MGI
abnormal epidermis stratum granulosum morphology / MGI
abnormal epidermis stratum corneum morphology / MGI
eyelids open at birth / MGI
excessive scratching / MGI
abnormal lipid level / MGI
decreased embryo size / MGI
abnormal dorsal-ventral axis patterning / MGI
anencephaly / MGI
neoplasm / MGI
abnormal neural tube morphology / MGI
no abnormal phenotype detected / MGI
delayed neural tube closure / MGI
abnormal keratinocyte differentiation / MGI
impaired skin barrier function / MGI
curly tail / MGI
spina bifida / MGI
abnormal neural tube closure / MGI
abnormal craniofacial development / MGI
fetal growth retardation / MGI
abnormal vertebral spinous process morphology / MGI
absent vertebral arch / MGI
split vertebrae / MGI
thick epidermis stratum spinosum / MGI
increased keratinocyte proliferation / MGI
abnormal corneocyte envelope morphology / MGI
abnormal palate development / MGI
perinatal lethality, complete penetrance / MGI
preweaning lethality, complete penetrance / MGI
lethality during fetal growth through weaning, complete penetrance / MGI
spina bifida cystica / MGI
abnormal periderm development / MGI
abnormal corneocyte morphology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Grhl3tm1a(EUCOMM)Wtsi/IcsOrl