STOCK Cdk4tm2.1Bbd/Cnbc
| Status | Available to order |
| EMMA ID | EM:08029 |
| International strain name | STOCK Cdk4tm2.1Bbd/Cnbc |
| Alternative name | Cdk4 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Cdk4tm2.1Bbd, |
| Gene/Transgene symbol | Cdk4 |
Information from provider
| Provider | Mariano Barbacid |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas |
| Genetic information | An frt site was inserted 230 bp upstream of exon 2 and a PGK-hygromycin cassette flanked by frt sites was inserted 115 bp downstream of exon 4. The PGK-hygromycin cassette and exons 2-4 of Cdk4 were excised by Flp-mediated recombination. This is the resulting knock-out mouse line, after frt-mediated exon excision. |
| Phenotypic information | Homozygous:Flp-mediated excision of exons 2-4 creates a null allele for Cdk4. Homozygous mice for the null allele have smaller size, noticeable at birth and progressively more noticeable with age. Mice develop diabetes after 2 months of age due to a drastic reduction in pancreatic beta cell numbers and islet size. Complete infertility in females and partial infertility in males is also observed. Reduction in pituitary gland size and pituitary-hormones production.Heterozygous:Heterozygous mice are viable |
| Breeding history | Chimeras were crossed with CD1 females for germ line transmission analysis. Heterozygous animals for the three-frt sites allele were crossed with transgenic CAG-Flp mice to excise the frt-PGKneo-frt cassette and exons 2-4 of Cdk4. Line was maintained by inter-crossing heterozygous animals for the Cdk4 null allele. Genetic background involves 129S1/Sv, 129X1/SvJ, CD1 and C57BL/6J. |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J |
Literature references
- Mice thrive without Cdk4 and Cdk2.;Barrière Cédric, Santamaría David, Cerqueira Antonio, Galán Javier, Martín Alberto, Ortega Sagrario, Malumbres Marcos, Dubus Pierre, Barbacid Mariano, ;2007;Molecular oncology;1;72-83; 19383288
- A synthetic lethal interaction between K-Ras oncogenes and Cdk4 unveils a therapeutic strategy for non-small cell lung carcinoma.;Puyol Marta, Martín Alberto, Dubus Pierre, Mulero Francisca, Pizcueta Pilar, Khan Gulfaraz, Guerra Carmen, Santamaría David, Barbacid Mariano, ;2010;Cancer cell;18;63-73; 20609353