B6NCrl;B6N-A^tm1Brd Il10ra^{tm1a(KOMP)Wtsi}/Orl

Status	Available to order
EMMA ID	EM:08265
Citation information	RRID:IMSR_EM:08265 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6NCrl;B6N-A^tm1Brd Il10ra^{tm1a(KOMP)Wtsi}/Orl
Alternative name	EPD0902_3_C05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Il10ra^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Il10ra
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Institut de Transgenose, INTRAGENE
Provider affiliation	TAAM-CDTA UPS44, Institut de Transgenose, INTRAGENE
Genetic information	This mouse line originates from KOMP ES clone EPD0902_3_C05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6NCrl males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Immune dysregulation-inflammatory bowel disease-arthritis-recurrent infections syndrome / Orphanet_238569

IMPC phenotypes (gene matching)

abnormal auditory brainstem response / IMPC
increased neutrophil cell number / IMPC
improved glucose tolerance / IMPC
decreased startle reflex / IMPC
decreased blood urea nitrogen level / IMPC
decreased lymphocyte cell number / IMPC

MGI phenotypes (gene matching)

increased IgG level / MGI
colitis / MGI
increased anti-chromatin antibody level / MGI
increased susceptibility to parasitic infection / MGI
increased susceptibility to induced colitis / MGI
increased circulating tumor necrosis factor level / MGI
increased circulating interferon-gamma level / MGI
increased circulating interleukin-6 level / MGI
increased circulating interleukin-2 level / MGI
increased circulating interleukin-17 level / MGI
increased circulating interleukin-12 level / MGI
increased circulating interleukin-1 alpha level / MGI
increased circulating interleukin-1 beta level / MGI
abnormal cytokine level / MGI
abnormal chemokine level / MGI
cecum inflammation / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6NCrl;B6N-Atm1Brd Il10ratm1a(KOMP)Wtsi/Orl