C57BL/6N-Fzd3^{tm1a(EUCOMM)Hmgu}/H

Status	Available to order
EMMA ID	EM:08277
Citation information	RRID:IMSR_EM:08277 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Fzd3^{tm1a(EUCOMM)Hmgu}/H
Alternative name	HEPD0675_6_G04
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Fzd3^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Fzd3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	MRC, Medical Research Council
Provider affiliation	Mary Lyon Centre at MRC Harwell
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0675_6_G04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)

decreased grip strength / IMPC
preweaning lethality, incomplete penetrance / IMPC
impaired glucose tolerance / IMPC
decreased bone mineral content / IMPC
increased total body fat amount / IMPC

MGI phenotypes (gene matching)

abnormal hindlimb morphology / MGI
abnormal nervous system tract morphology / MGI
decreased corpus callosum size / MGI
abnormal telencephalon morphology / MGI
dilated lateral ventricles / MGI
dilated third ventricle / MGI
incomplete rostral neuropore closure / MGI
abnormal spinal cord morphology / MGI
abnormal lung morphology / MGI
abnormal vocalization / MGI
cyanosis / MGI
abnormal breathing pattern / MGI
no abnormal phenotype detected / MGI
reduced sensorimotor gating / MGI
curly tail / MGI
abnormal depression-related behavior / MGI
abnormal striatum morphology / MGI
growth/size/body region phenotype / MGI
cellular phenotype / MGI
thin cerebral cortex / MGI
absent brain internal capsule / MGI
absent anterior commissure / MGI
enlarged lateral ventricles / MGI
decreased prepulse inhibition / MGI
impaired cued conditioning behavior / MGI
neonatal lethality, complete penetrance / MGI
decreased striatum area / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Fzd3tm1a(EUCOMM)Hmgu/H