B6D2-Tg(Kit-EGFP)3Poz/Cnrm

Status	Available to order
EMMA ID	EM:08339
International strain name	B6D2-Tg(Kit-EGFP)3Poz/Cnrm
Alternative name	c-kit/GFP transgenic line 3
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Kit-EGFP)3Poz
Gene/Transgene symbol	Tg(Kit-EGFP)3Poz

Information from provider

Provider	Sergio Ottolenghi
Provider affiliation	Biotecnologie e Bioscienze, Università Milano-Bicocca
Genetic information	A GFP-expressing transgenic line, in which GFP expression is driven by a mouse c-kit promoter/enhancer construct.
Phenotypic information	Homozygous: No specific phenotype; GFP expression in c-kit-positive cells (hematopoietic stem/progenitors, primordial germ cells and some spermatogonia, cardiac stem cells). Heterozygous: no specific phenotype; GFP expression in c-kit-positive cells (hematopoietic stem/progenitors, primordial germ cells and some spermatogonia, cardiac stem cells).
Breeding history	The line was sib to sib crossed for at least 25 generations, but also crossed 3 times to non transgenic (C57BL/6 x DBA/2)F1 mice.
References	Kit regulatory elements required for expression in developing hematopoietic and germ cell lineages.;Cairns Linda A, Moroni Emanuela, Levantini Elena, Giorgetti Alessandra, Klinger Francesca G, Ronzoni Simona, Tatangelo Laura, Tiveron Cecilia, De Felici Massimo, Dolci Susanna, Magli Maria Cristina, Giglioni Barbara, Ottolenghi Sergio, ;2003;Blood;102;3954-62; 12907433 Stem cell-mediated muscle regeneration is enhanced by local isoform of insulin-like growth factor 1.;Musarò Antonio, Giacinti Cristina, Borsellino Giovanna, Dobrowolny Gabriella, Pelosi Laura, Cairns Linda, Ottolenghi Sergio, Cossu Giulio, Bernardi Giorgio, Battistini Luca, Molinaro Mario, Rosenthal Nadia, ;2004;Proceedings of the National Academy of Sciences of the United States of America;101;1206-10; 14745025 Repression of kit expression by Plzf in germ cells.;Filipponi Doria, Hobbs Robin M, Ottolenghi Sergio, Rossi Pellegrino, Jannini Emmanuele A, Pandolfi Pier Paolo, Dolci Susanna, ;2007;Molecular and cellular biology;27;6770-81; 17664282 Green fluorescent protein transgene driven by Kit regulatory sequences is expressed in hematopoietic stem cells.;Cerisoli Francesco, Cassinelli Letizia, Lamorte Giuseppe, Citterio Stefania, Bertolotti Francesca, Magli Maria Cristina, Ottolenghi Sergio, ;2009;Haematologica;94;318-25; 19181779 Spontaneous calcium oscillations regulate human cardiac progenitor cell growth.;Ferreira-Martins João, Rondon-Clavo Carlos, Tugal Derin, Korn Justin A, Rizzi Roberto, Padin-Iruegas Maria Elena, Ottolenghi Sergio, De Angelis Antonella, Urbanek Konrad, Ide-Iwata Noriko, D'Amario Domenico, Hosoda Toru, Leri Annarosa, Kajstura Jan, Anversa Piero, Rota Marcello, ;2009;Circulation research;105;764-74; 19745162 Bone marrow-derived cells can acquire cardiac stem cells properties in damaged heart.;Barile Lucio, Cerisoli Francesco, Frati Giacomo, Gaetani Roberto, Chimenti Isotta, Forte Elvira, Cassinelli Letizia, Spinardi Laura, Altomare Claudia, Kizana Eddy, Giacomello Alessandro, Messina Elisa, Ottolenghi Sergio, Magli Maria Cristina, ;2011;Journal of cellular and molecular medicine;15;63-71; 19912439 Cardiomyogenesis in the developing heart is regulated by c-kit-positive cardiac stem cells.;Ferreira-Martins João, Ogórek Barbara, Cappetta Donato, Matsuda Alex, Signore Sergio, D'Amario Domenico, Kostyla James, Steadman Elisabeth, Ide-Iwata Noriko, Sanada Fumihiro, Iaffaldano Grazia, Ottolenghi Sergio, Hosoda Toru, Leri Annarosa, Kajstura Jan, Anversa Piero, Rota Marcello, ;2012;Circulation research;110;701-15; 22275487 SOHLH1 and SOHLH2 control Kit expression during postnatal male germ cell development.;Barrios Florencia, Filipponi Doria, Campolo Federica, Gori Manuele, Bramucci Federica, Pellegrini Manuela, Ottolenghi Sergio, Rossi Pellegrino, Jannini Emmanuele A, Dolci Susanna, ;2012;Journal of cell science;125;1455-64; 22328502 Adult c-kit(pos) cardiac stem cells are necessary and sufficient for functional cardiac regeneration and repair.;Ellison Georgina M, Vicinanza Carla, Smith Andrew J, Aquila Iolanda, Leone Angelo, Waring Cheryl D, Henning Beverley J, Stirparo Giuliano Giuseppe, Papait Roberto, Scarfò Marzia, Agosti Valter, Viglietto Giuseppe, Condorelli Gianluigi, Indolfi Ciro, Ottolenghi Sergio, Torella Daniele, Nadal-Ginard Bernardo, ;2013;Cell;154;827-42; 23953114 The ephrin A1-EphA2 system promotes cardiac stem cell migration after infarction.;Goichberg Polina, Bai Yingnan, D'Amario Domenico, Ferreira-Martins João, Fiorini Claudia, Zheng Hanqiao, Signore Sergio, del Monte Federica, Ottolenghi Sergio, D'Alessandro David A, Michler Robert E, Hosoda Toru, Anversa Piero, Kajstura Jan, Rota Marcello, Leri Annarosa, ;2011;Circulation research;108;1071-83; 21415392
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Literature references

Kit regulatory elements required for expression in developing hematopoietic and germ cell lineages.;Cairns Linda A, Moroni Emanuela, Levantini Elena, Giorgetti Alessandra, Klinger Francesca G, Ronzoni Simona, Tatangelo Laura, Tiveron Cecilia, De Felici Massimo, Dolci Susanna, Magli Maria Cristina, Giglioni Barbara, Ottolenghi Sergio, ;2003;Blood;102;3954-62; 12907433
Stem cell-mediated muscle regeneration is enhanced by local isoform of insulin-like growth factor 1.;Musarò Antonio, Giacinti Cristina, Borsellino Giovanna, Dobrowolny Gabriella, Pelosi Laura, Cairns Linda, Ottolenghi Sergio, Cossu Giulio, Bernardi Giorgio, Battistini Luca, Molinaro Mario, Rosenthal Nadia, ;2004;Proceedings of the National Academy of Sciences of the United States of America;101;1206-10; 14745025
Repression of kit expression by Plzf in germ cells.;Filipponi Doria, Hobbs Robin M, Ottolenghi Sergio, Rossi Pellegrino, Jannini Emmanuele A, Pandolfi Pier Paolo, Dolci Susanna, ;2007;Molecular and cellular biology;27;6770-81; 17664282
Green fluorescent protein transgene driven by Kit regulatory sequences is expressed in hematopoietic stem cells.;Cerisoli Francesco, Cassinelli Letizia, Lamorte Giuseppe, Citterio Stefania, Bertolotti Francesca, Magli Maria Cristina, Ottolenghi Sergio, ;2009;Haematologica;94;318-25; 19181779
Spontaneous calcium oscillations regulate human cardiac progenitor cell growth.;Ferreira-Martins João, Rondon-Clavo Carlos, Tugal Derin, Korn Justin A, Rizzi Roberto, Padin-Iruegas Maria Elena, Ottolenghi Sergio, De Angelis Antonella, Urbanek Konrad, Ide-Iwata Noriko, D'Amario Domenico, Hosoda Toru, Leri Annarosa, Kajstura Jan, Anversa Piero, Rota Marcello, ;2009;Circulation research;105;764-74; 19745162
Bone marrow-derived cells can acquire cardiac stem cells properties in damaged heart.;Barile Lucio, Cerisoli Francesco, Frati Giacomo, Gaetani Roberto, Chimenti Isotta, Forte Elvira, Cassinelli Letizia, Spinardi Laura, Altomare Claudia, Kizana Eddy, Giacomello Alessandro, Messina Elisa, Ottolenghi Sergio, Magli Maria Cristina, ;2011;Journal of cellular and molecular medicine;15;63-71; 19912439
Cardiomyogenesis in the developing heart is regulated by c-kit-positive cardiac stem cells.;Ferreira-Martins João, Ogórek Barbara, Cappetta Donato, Matsuda Alex, Signore Sergio, D'Amario Domenico, Kostyla James, Steadman Elisabeth, Ide-Iwata Noriko, Sanada Fumihiro, Iaffaldano Grazia, Ottolenghi Sergio, Hosoda Toru, Leri Annarosa, Kajstura Jan, Anversa Piero, Rota Marcello, ;2012;Circulation research;110;701-15; 22275487
SOHLH1 and SOHLH2 control Kit expression during postnatal male germ cell development.;Barrios Florencia, Filipponi Doria, Campolo Federica, Gori Manuele, Bramucci Federica, Pellegrini Manuela, Ottolenghi Sergio, Rossi Pellegrino, Jannini Emmanuele A, Dolci Susanna, ;2012;Journal of cell science;125;1455-64; 22328502
Adult c-kit(pos) cardiac stem cells are necessary and sufficient for functional cardiac regeneration and repair.;Ellison Georgina M, Vicinanza Carla, Smith Andrew J, Aquila Iolanda, Leone Angelo, Waring Cheryl D, Henning Beverley J, Stirparo Giuliano Giuseppe, Papait Roberto, Scarfò Marzia, Agosti Valter, Viglietto Giuseppe, Condorelli Gianluigi, Indolfi Ciro, Ottolenghi Sergio, Torella Daniele, Nadal-Ginard Bernardo, ;2013;Cell;154;827-42; 23953114
The ephrin A1-EphA2 system promotes cardiac stem cell migration after infarction.;Goichberg Polina, Bai Yingnan, D'Amario Domenico, Ferreira-Martins João, Fiorini Claudia, Zheng Hanqiao, Signore Sergio, del Monte Federica, Ottolenghi Sergio, D'Alessandro David A, Michler Robert E, Hosoda Toru, Anversa Piero, Kajstura Jan, Rota Marcello, Leri Annarosa, ;2011;Circulation research;108;1071-83; 21415392

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