B6.Cg-Tg(Nr5a1-CHRM4*,-tdTomato)1Cogr/Cnrm
| Status | Available to order |
| EMMA ID | EM:08358 |
| International strain name | B6.Cg-Tg(Nr5a1-CHRM4*,-tdTomato)1Cogr/Cnrm |
| Alternative name | Nr5a1::hM4D-2A-TomatoF |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Nr5a1-CHRM4*, -tdTomato)1Cogr |
| Gene/Transgene symbol | Tg(Nr5a1-CHRM4*,-tdTomato)1Cogr |
Information from provider
| Provider | Cornelius Gross |
| Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
| Genetic information | The Nr5a1::hM4D-2A-TomatoF BAC transgenic mouse line was obtained inserting the hM4D-2A-TomatoF cassette at the ATG site of the Nr5a1 gene in the Nr5a1 containing mouse BAC RP23225F7. |
| Phenotypic information | Homozygous:Mice hemizygous for the Nr5a1::hM4D-2A-TomatoF BAC transgene are viable and fertile, with expression of the pharmacogenetic inhibitory receptor hM4D (Armbruster et al. 2007) and farnesylated Tomato in the dorsomedial portion of the ventromedial hypothalamic nucleus (VMHdm). The expression of hM4D and TomatoF is restricted to Nr5a1 expressing cells. The hM4D is a modified version of the human muscarinic receptor M4 with high affinity for the otherwise biologically inert drug Clozapine-N-Oxide (CNO), and virtually no affinity to its endogenous ligand acetylcholine. Activation of the hM4D by CNO in neurons induces a decrease in membrane potential and neuronal firing and was used to selectively and rapidly inhibit neurons in the VMHdm in behaving animals. The expression of the reporter gene TomatoF marks the VMHdm and its projections to all its known target areas.Heterozygous:Mice hemizygous for the Nr5a1::hM4D-2A-TomatoF BAC transgene are viable and fertile, with expression of the pharmacogenetic inhibitory receptor hM4D (Armbruster et al. 2007) and farnesylated Tomato in the dorsomedial portion of the ventromedial hypothalamic nucleus (VMHdm). The expression of hM4D and TomatoF is restricted to Nr5a1 expressing cells. The hM4D is a modified version of the human muscarinic receptor M4 with high affinity for the otherwise biologically inert drug Clozapine-N-Oxide (CNO), and virtually no affinity to its endogenous ligand acetylcholine. Activation of the hM4D by CNO in neurons induces a decrease in membrane potential and neuronal firing and was used to selectively and rapidly inhibit neurons in the VMHdm in behaving animals. The expression of the reporter gene TomatoF marks the VMHdm and its projections to all its known target areas |
| Breeding history | 10 backcrosses with C57BL/6N. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Independent hypothalamic circuits for social and predator fear.;Silva Bianca A, Mattucci Camilla, Krzywkowski Piotr, Murana Emanuele, Illarionova Anna, Grinevich Valery, Canteras Newton S, Ragozzino Davide, Gross Cornelius T, ;2013;Nature neuroscience;16;1731-3; 24212674