C57BL/6NTac-Gm5544tm1a(KOMP)Wtsi/WtsiOrl

Status

Available to order

EMMA IDEM:08391
Citation informationRRID:IMSR_EM:08391 

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International strain nameC57BL/6NTac-Gm5544tm1a(KOMP)Wtsi/WtsiOrl
Alternative nameEPD0164_6_A12
Strain typeTargeted Mutant Strains
Allele/Transgene symbolGm5544tm1a(KOMP)Wtsi
Gene/Transgene symbolGm5544
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from KOMP ES clone EPD0164_6_A12. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous C57BL/6NTac males

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased marginal zone B cell number / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
IMPC phenotypes (gene matching)
  • decreased marginal zone B cell number / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
MGI phenotypes (allele matching)
  • abnormal basisphenoid bone morphology / MGI
  • polydactyly / MGI
  • exencephaly / MGI
  • small kidney / MGI
  • abnormal eye muscle morphology / MGI
  • occipital bone foramen / MGI
  • basisphenoid bone foramen / MGI
  • fusion of vertebral arches / MGI
  • abnormal pericardial cavity morphology / MGI
  • abnormal intrathoracic topology of vagus nerve / MGI
  • abnormal hypoglossal nerve topology / MGI
  • absent segment of posterior cerebral artery / MGI
  • subcutaneous edema / MGI
  • blood in lymph vessels / MGI
  • abnormal umbilical vein topology / MGI
  • embryo tumor / MGI
MGI phenotypes (gene matching)
  • abnormal basisphenoid bone morphology / MGI
  • polydactyly / MGI
  • exencephaly / MGI
  • small kidney / MGI
  • abnormal eye muscle morphology / MGI
  • occipital bone foramen / MGI
  • basisphenoid bone foramen / MGI
  • fusion of vertebral arches / MGI
  • abnormal pericardial cavity morphology / MGI
  • abnormal intrathoracic topology of vagus nerve / MGI
  • abnormal hypoglossal nerve topology / MGI
  • absent segment of posterior cerebral artery / MGI
  • subcutaneous edema / MGI
  • blood in lymph vessels / MGI
  • abnormal umbilical vein topology / MGI
  • embryo tumor / MGI

Information on how we integrate external resources can be found here

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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