C57BL/6N-A^tm1Brd Tmem127^{tm1(KOMP)Wtsi}/WtsiOulu

Status	Available to order
EMMA ID	EM:08447
Citation information	RRID:IMSR_EM:08447 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-A^tm1Brd Tmem127^{tm1(KOMP)Wtsi}/WtsiOulu
Alternative name	EPD0406_4_C10
Strain type
Allele/Transgene symbol	Tmem127^{tm1(KOMP)Wtsi}
Gene/Transgene symbol	Tmem127
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0406_4_C10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased neutrophil cell number / IMPC
decreased monocyte cell number / IMPC
abnormal nail morphology / IMPC
tremors / IMPC
increased circulating alkaline phosphatase level / IMPC
thrombocytopenia / IMPC
increased T cell number / IMPC
decreased B cell number / IMPC
increased circulating bilirubin level / IMPC
increased circulating creatinine level / IMPC
decreased NK cell number / IMPC
increased CD4-positive, alpha-beta T cell number / IMPC
decreased CD4-positive, alpha-beta T cell number / IMPC
increased CD8-positive, alpha-beta T cell number / IMPC
decreased plasmacytoid dendritic cell number / IMPC
decreased B-2 B cell number / IMPC
increased circulating amylase level / IMPC
increased bone mineral content / IMPC
decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
increased alpha-beta T cell number / IMPC
increased KLRG1-positive NK cell number / IMPC
decreased KLRG1-positive NK cell number / IMPC
obsolete increased CD8-positive, alpha beta T cell number / IMPC
decreased Ly6C high monocyte number / IMPC
increased Ly6C low monocyte number / IMPC
decreased memory-marker NK cell number / IMPC
increased memory-marker NK cell number / IMPC
increased CD8-positive, naive alpha-beta T cell number / IMPC
decreased KLRG1-positive T-helper cell number / IMPC

IMPC phenotypes (gene matching)

increased CD8-positive, alpha-beta T cell number / IMPC
increased alpha-beta T cell number / IMPC
increased circulating alkaline phosphatase level / IMPC
decreased neutrophil cell number / IMPC
decreased CD4-positive, alpha-beta T cell number / IMPC
decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
decreased plasmacytoid dendritic cell number / IMPC
increased T cell number / IMPC
decreased KLRG1-positive T-helper cell number / IMPC
tremors / IMPC
increased circulating amylase level / IMPC
decreased B cell number / IMPC
increased memory-marker NK cell number / IMPC
obsolete increased CD8-positive, alpha beta T cell number / IMPC
increased bone mineral content / IMPC
decreased monocyte cell number / IMPC
thrombocytopenia / IMPC
increased CD8-positive, naive alpha-beta T cell number / IMPC
increased KLRG1-positive NK cell number / IMPC
decreased NK cell number / IMPC
increased CD4-positive, alpha-beta T cell number / IMPC
decreased B-2 B cell number / IMPC
abnormal nail morphology / IMPC
increased Ly6C low monocyte number / IMPC
decreased KLRG1-positive NK cell number / IMPC
decreased Ly6C high monocyte number / IMPC
increased circulating creatinine level / IMPC
decreased memory-marker NK cell number / IMPC
increased circulating bilirubin level / IMPC

MGI phenotypes (gene matching)

abnormal cell morphology / MGI
no phenotypic analysis / MGI
abnormal lysosome physiology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Tmem127tm1(KOMP)Wtsi/WtsiOulu