C57BL/6N-A^tm1Brd Cert1^{tm1a(KOMP)Wtsi}/WtsiFlmg

Status	Available to order
EMMA ID	EM:08488
Citation information	RRID:IMSR_EM:08488 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-A^tm1Brd Cert1^{tm1a(KOMP)Wtsi}/WtsiFlmg
Alternative name	EPD0855_2_H11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cert1^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Cert1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0855_2_H11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased mean corpuscular hemoglobin / IMPC
preweaning lethality, incomplete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, incomplete penetrance / IMPC
decreased mean corpuscular hemoglobin / IMPC

MGI phenotypes (gene matching)

abnormal heart morphology / MGI
thin ventricular wall / MGI
double outlet right ventricle / MGI
decreased cell proliferation / MGI
abnormal muscle development / MGI
abnormal forebrain morphology / MGI
abnormal cardiovascular system physiology / MGI
decreased embryo size / MGI
abnormal lipid homeostasis / MGI
abnormal nasal cavity morphology / MGI
abnormal semicircular canal morphology / MGI
persistent truncus arteriosis / MGI
absent tibia / MGI
no phenotypic analysis / MGI
aphakia / MGI
fetal growth retardation / MGI
abnormal optic cup morphology / MGI
abnormal vertebral arch morphology / MGI
fusion of vertebral bodies / MGI
fusion of vertebral arches / MGI
decreased cervical vertebrae number / MGI
homeostasis/metabolism phenotype / MGI
cellular phenotype / MGI
reproductive system phenotype / MGI
decreased ventricle muscle contractility / MGI
abnormal cell physiology / MGI
abnormal mitochondrion morphology / MGI
abnormal mitochondrial physiology / MGI
abnormal cardiac outflow tract development / MGI
increased heart ventricle size / MGI
abnormal cervical rib / MGI
abnormal endoplasmic reticulum morphology / MGI
abnormal cardiac jelly morphology / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
abnormal pectinate muscle morphology / MGI
persistent right dorsal aorta / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
abnormal umbilical vein topology / MGI
abnormal vertebral artery topology / MGI
heterochrony / MGI
persistent trigeminal artery / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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