B6;129-Tdrd9tm1Embrp/Cnrm
| Status | Available to order |
| EMMA ID | EM:08489 |
| International strain name | B6;129-Tdrd9tm1Embrp/Cnrm |
| Alternative name | Tdrd9 KI (E257Q) cKO+Neo. |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Tdrd9tm1Embrp, |
| Gene/Transgene symbol | Tdrd9 |
Information from provider
| Provider | Ramesh PILLAI |
| Provider affiliation | Grenoble Outstation, EMBL |
| Genetic information | The Tdrd9 gene carries a single point mutation (E257Q) in the ATPase motif (exon 5) that is predicted to kill its activity. Presence of loxP sites flanking exons 3-5 allows generation of conditional KOs. Note that the Neo cassette used for selection of the targeted ES cell line (A9 ES cells) is retained in the mouse line. |
| Phenotypic information | Homozygous:Homozygous KI males are infertile.Heterozygous:Males and females are viable and fertile. |
| Breeding history | The founder male was generated with targeted A9 ES cells injected into C57BL/6N host embryos. The founder was crossed with C57BL/6N females. The F1 generation was genotyped for presence of the targeted allele. |
| References |
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| Homozygous fertile | females only |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Male infertility with azoospermia or oligozoospermia due to single gene mutation / Orphanet_399805
Literature references
- Distinct Roles of RNA Helicases MVH and TDRD9 in PIWI Slicing-Triggered Mammalian piRNA Biogenesis and Function.;Wenda Joanna M, Homolka David, Yang Zhaolin, Spinelli Pietro, Sachidanandam Ravi, Pandey Radha Raman, Pillai Ramesh S, ;2017;Developmental cell;41;623-637.e9; 28633017