C57BL/6N-Dnase1l2tm1.1(KOMP)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:08574
International strain nameC57BL/6N-Dnase1l2tm1.1(KOMP)Wtsi/WtsiH
Alternative nameEPD0296_2_A10
Strain typeTargeted Mutant Strains
Allele/Transgene symbolDnase1l2tm1.1(KOMP)Wtsi
Gene/Transgene symbolDnase1l2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from KOMP ES clone EPD0296_2_A10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased bone mineral density / IMPC
  • abnormal head morphology / IMPC
  • abnormal cranium morphology / IMPC
  • abnormal maxilla morphology / IMPC
  • abnormal stomach morphology / IMPC
  • abnormal tibia morphology / IMPC
  • abnormal autopod morphology / IMPC
  • abnormal nail morphology / IMPC
  • abnormal spleen morphology / IMPC
  • decreased body length / IMPC
  • abnormal lens morphology / IMPC
  • cataract / IMPC
  • hypoactivity / IMPC
  • decreased startle reflex / IMPC
  • abnormal vocalization / IMPC
  • increased circulating HDL cholesterol level / IMPC
  • abnormal motor capabilities/coordination/movement / IMPC
  • abnormal eye morphology / IMPC
  • abnormal digit morphology / IMPC
  • abnormal tail morphology / IMPC
  • brachydactyly / IMPC
  • decreased circulating insulin level / IMPC
  • short tibia / IMPC
  • abnormal joint morphology / IMPC
  • abnormal bone structure / IMPC
  • abnormal heart left ventricle morphology / IMPC
  • increased lean body mass / IMPC
  • decreased lean body mass / IMPC
  • abnormal spine curvature / IMPC
  • vertebral fusion / IMPC
  • fusion of vertebral arches / IMPC
  • abnormal behavior / IMPC
  • decreased circulating glucose level / IMPC
  • increased blood urea nitrogen level / IMPC
  • abnormal optic disk morphology / IMPC
  • increased circulating amylase level / IMPC
  • decreased total body fat amount / IMPC
  • increased grip strength / IMPC
  • decreased grip strength / IMPC
  • decreased bone mineral content / IMPC
  • decreased food intake / IMPC
  • increased fasted circulating glucose level / IMPC

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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