B6;FVB-Tg(tetO-GFP)2Jstr/Flmg

Status	Available to order
EMMA ID	EM:09009
Citation information	RRID:IMSR_EM:09009 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;FVB-Tg(tetO-GFP)2Jstr/Flmg
Alternative name	B6.FVB-Tg(tetO-GFP)2Jstr/Flmg
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(tetO-GFP)2Jstr
Gene/Transgene symbol	Tg(tetO-GFP)2Jstr

Information from provider

Provider	John Strouboulis
Provider affiliation	Molecular Oncology, BSRC Al. Fleming
Additional owner	Frank Grosveld, Department of Cell Biology, Erasmus University Medical Center, Rotterdam, The Netherlands
Genetic information	The tetracycline operator drives GFP expression in the presence of doxycycline. Three transgenic lines carrying the Tet-inducible TRE-GFP reporter construct were generated by microinjection. Analysis of GFP expression in the liver of adult mice treated with Dox showed that TRE-GFP line 2 was induced more efficiently than TRE-GFP lines 1 and 3. Thus, TRE-GFP line 2 was retained and used as a reporter line in further studies.
Phenotypic information	Homozygous: no overt phenotype Heterozygous: no overt phenotype
References	Ubiquitous expression of the rtTA2S-M2 inducible system in transgenic mice driven by the human hnRNPA2B1/CBX3 CpG island.;Katsantoni Eleni Z, Anghelescu Nora E, Rottier Robbert, Moerland Matthijs, Antoniou Michael, de Crom Rini, Grosveld Frank, Strouboulis John, ;2007;BMC developmental biology;7;108; 17900353
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archiving	heterozygous FVB/N x C57BL/6 males

Literature references

Ubiquitous expression of the rtTA2S-M2 inducible system in transgenic mice driven by the human hnRNPA2B1/CBX3 CpG island.;Katsantoni Eleni Z, Anghelescu Nora E, Rottier Robbert, Moerland Matthijs, Antoniou Michael, de Crom Rini, Grosveld Frank, Strouboulis John, ;2007;BMC developmental biology;7;108; 17900353

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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