C57BL/6N-Ap4e1tm1c(KOMP)Wtsi/H

Status

Available to order

EMMA IDEM:09035
Citation informationRRID:IMSR_EM:09035 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Ap4e1tm1c(KOMP)Wtsi/H
Alternative nameEPD0025_5_A08
Strain typeTargeted Mutant Strains : Targeted Conditional
Allele/Transgene symbolAp4e1tm1c(KOMP)Wtsi
Gene/Transgene symbolAp4e1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis line originates from KOMP ES clone EPD0025_5_A08, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

    • Severe intellectual disability and progressive spastic paraplegia / Orphanet_280763
IMPC phenotypes (gene matching)
  • abnormal testis morphology / IMPC
  • abnormal lens morphology / IMPC
  • decreased total retina thickness / IMPC
  • decreased mean corpuscular hemoglobin concentration / IMPC
  • abnormal spleen morphology / IMPC
  • increased lean body mass / IMPC
  • hyperactivity / IMPC
  • decreased heart rate / IMPC
  • increased startle reflex / IMPC
  • decreased grip strength / IMPC
  • abnormal vertebral arch morphology / IMPC
  • increased grip strength / IMPC
  • hyperplasia / IMPC
  • increased circulating sodium level / IMPC
  • decreased exploration in new environment / IMPC
  • decreased total body fat amount / IMPC
  • decreased circulating chloride level / IMPC
  • decreased vertical activity / IMPC
  • increased bone mineral content / IMPC
  • abnormal cholesterol homeostasis / IMPC
  • abnormal retina inner nuclear layer morphology / IMPC
  • decreased circulating serum albumin level / IMPC
  • decreased mean corpuscular volume / IMPC
  • decreased circulating glucose level / IMPC
  • increased prepulse inhibition / IMPC
  • fibro-osseous lesion / IMPC
  • increased lung compliance / IMPC
  • decreased respiratory quotient / IMPC
  • abnormal sleep behavior / IMPC
  • unresponsive to tactile stimuli / IMPC
  • increased cardiac muscle contractility / IMPC
  • abnormal behavior / IMPC
  • increased circulating creatine kinase level / IMPC
  • limb grasping / IMPC
  • increased circulating free fatty acids level / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • decreased thigmotaxis / IMPC
  • hypoplasia / IMPC
  • abnormal coat appearance / IMPC
  • increased kidney weight / IMPC
  • decreased circulating total protein level / IMPC
  • increased erythrocyte cell number / IMPC
  • decreased mean corpuscular hemoglobin / IMPC
  • abnormal inspiratory capacity / IMPC
  • impaired glucose tolerance / IMPC
  • small testis / IMPC
  • increased circulating amylase level / IMPC
  • decreased anxiety-related response / IMPC
  • decreased circulating triglyceride level / IMPC
  • abnormal vertebrae morphology / IMPC
  • cardiovascular system phenotype / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • decreased cardiac output / IMPC
  • decreased heart rate variability / IMPC
  • increased bone mineral density / IMPC
  • abnormal freezing behavior / IMPC
  • abnormal sinus arrhythmia / IMPC
  • decreased circulating alanine transaminase level / IMPC
  • depletion / IMPC
  • enlarged lymph nodes / IMPC
  • increased hemoglobin content / IMPC
  • enlarged spleen / IMPC
  • process of degenerative change / IMPC
  • increased red blood cell distribution width / IMPC
  • decreased locomotor activity / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • increased heart weight / IMPC
  • increased hematocrit / IMPC
  • impaired contextual conditioning behavior / IMPC
  • decreased circulating creatinine level / IMPC
  • decreased circulating free fatty acids level / IMPC
  • abnormal kidney morphology / IMPC
  • decreased hemoglobin content / IMPC
  • increased exploration in new environment / IMPC
  • increased fasting circulating glucose level / IMPC
MGI phenotypes (gene matching)
  • increased bone mineral density / MGI
  • abnormal femur morphology / MGI
  • decreased corpus callosum size / MGI
  • anemia / MGI
  • decreased vertical activity / MGI
  • decreased hemoglobin content / MGI
  • increased lean body mass / MGI
  • abnormal behavior / MGI
  • decreased mean corpuscular hemoglobin / MGI
  • enlarged lateral ventricles / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

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