C57BL/6N-Ngfrtm1c(EUCOMM)Wtsi/H

Status

Available to order

EMMA IDEM:09040
Citation informationRRID:IMSR_EM:09040 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Ngfrtm1c(EUCOMM)Wtsi/H
Alternative nameEPD0059_4_D03
Strain typeTargeted Mutant Strains : Targeted Conditional
Allele/Transgene symbolNgfrtm1c(EUCOMM)Wtsi
Gene/Transgene symbolNgfr
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider MRC, Medical Research Council
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis line originates from EUCOMM ES clone EPD0059_4_D03, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • preweaning lethality, incomplete penetrance / IMPC
  • abnormal digit morphology / IMPC
  • abnormal eye morphology / IMPC
  • increased startle reflex / IMPC
  • limb grasping / IMPC
  • abnormal gait / IMPC
  • decreased circulating triglyceride level / IMPC
  • decreased thigmotaxis / IMPC
  • abnormal retina morphology / IMPC
  • increased circulating phosphate level / IMPC
  • tremors / IMPC
  • abnormal behavior / IMPC
  • decreased circulating alkaline phosphatase level / IMPC
  • decreased locomotor activity / IMPC
MGI phenotypes (gene matching)
  • organ of Corti degeneration / MGI
  • absent hair follicles / MGI
  • abnormal mouth morphology / MGI
  • ulcerated paws / MGI
  • abnormal tongue epithelium morphology / MGI
  • abnormal superior colliculus morphology / MGI
  • decreased motor neuron number / MGI
  • abnormal dorsal root ganglion morphology / MGI
  • abnormal sensory neuron innervation pattern / MGI
  • small L4 dorsal root ganglion / MGI
  • abnormal L5 dorsal root ganglion morphology / MGI
  • abnormal cholinergic neuron morphology / MGI
  • abnormal innervation pattern to muscle / MGI
  • abnormal trigeminal ganglion morphology / MGI
  • decreased Schwann cell number / MGI
  • decreased body size / MGI
  • ataxia / MGI
  • abnormal gait / MGI
  • abnormal spatial learning / MGI
  • abnormal posture / MGI
  • limb grasping / MGI
  • abnormal blood vessel morphology / MGI
  • postnatal growth retardation / MGI
  • hemorrhage / MGI
  • abnormal sensory capabilities/reflexes/nociception / MGI
  • abnormal tooth morphology / MGI
  • abnormal muscle morphology / MGI
  • no abnormal phenotype detected / MGI
  • decreased airway responsiveness / MGI
  • abnormal sciatic nerve morphology / MGI
  • abnormal molar morphology / MGI
  • cochlear ganglion degeneration / MGI
  • abnormal neuron morphology / MGI
  • abnormal axon guidance / MGI
  • decreased myocardial infarction size / MGI
  • extremity edema / MGI
  • abnormal neuron apoptosis / MGI
  • decreased neuron apoptosis / MGI
  • abnormal axon extension / MGI
  • abnormal molar crown morphology / MGI
  • dilated dorsal aorta / MGI
  • abnormal peripheral nervous system regeneration / MGI
  • abnormal proprioceptive neuron morphology / MGI
  • cochlear hair cell degeneration / MGI
  • sensorineural hearing loss / MGI
  • increased susceptibility to age-related hearing loss / MGI
  • increased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • abnormal vascular wound healing / MGI
  • abnormal eye physiology / MGI
  • renal/urinary system phenotype / MGI
  • increased retinal photoreceptor cell number / MGI
  • abnormal circumvallate papillae morphology / MGI
  • abnormal gustatory papillae taste bud morphology / MGI
  • abnormal retinal ganglion cell morphology / MGI
  • abnormal Kupffer cell morphology / MGI
  • decreased muscle spindle number / MGI
  • abnormal tumor necrosis factor level / MGI
  • increased neuron number / MGI
  • abnormal axon pruning / MGI
  • abnormal neuron differentiation / MGI
  • perinatal lethality, incomplete penetrance / MGI
  • increased or absent threshold for auditory brainstem response / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.​
Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).