C57BL/6N-Cyp7a1tm1a(EUCOMM)Wtsi/Ieg

Status

Available to order

EMMA IDEM:09086
Citation informationRRID:IMSR_EM:09086 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Cyp7a1tm1a(EUCOMM)Wtsi/Ieg
Alternative nameEPD0426_3_C04
Strain typeTargeted Mutant Strains
Allele/Transgene symbolCyp7a1tm1a(EUCOMM)Wtsi
Gene/Transgene symbolCyp7a1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliationInstitute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0426_3_C04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6NTac (USA) males, wild-type C57BL/6NTac (USA) females
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

    • Hypercholesterolemia due to cholesterol 7alpha-hydroxylase deficiency / Orphanet_209902
IMPC phenotypes (gene matching)
  • increased circulating HDL cholesterol level / IMPC
  • abnormal retina blood vessel morphology / IMPC
  • increased startle reflex / IMPC
  • abnormal retina vasculature morphology / IMPC
  • decreased circulating serum albumin level / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • abnormal retina morphology / IMPC
  • persistence of hyaloid vascular system / IMPC
  • increased circulating bilirubin level / IMPC
  • abnormal lens morphology / IMPC
  • decreased circulating iron level / IMPC
  • abnormal vitreous body morphology / IMPC
  • decreased blood urea nitrogen level / IMPC
  • cataract / IMPC
  • increased leukocyte cell number / IMPC
  • decreased exploration in new environment / IMPC
  • increased circulating cholesterol level / IMPC
  • increased circulating unsaturated transferrin level / IMPC
MGI phenotypes (gene matching)
  • abnormal liver morphology / MGI
  • abnormal liver physiology / MGI
  • abnormal skin condition / MGI
  • flaky skin / MGI
  • oily skin / MGI
  • abnormal epidermis stratum basale morphology / MGI
  • thin epidermis stratum spinosum / MGI
  • abnormal epidermis stratum granulosum morphology / MGI
  • hyperkeratosis / MGI
  • thin dermal layer / MGI
  • delayed eyelid opening / MGI
  • hypoactivity / MGI
  • decreased exploration in new environment / MGI
  • postnatal growth retardation / MGI
  • abnormal skin morphology / MGI
  • abnormal vision / MGI
  • abnormal lipid homeostasis / MGI
  • decreased intestinal cholesterol absorption / MGI
  • steatorrhea / MGI
  • greasy coat / MGI
  • dry skin / MGI
  • abnormal bile composition / MGI
  • abnormal bile salt level / MGI
  • abnormal bile salt homeostasis / MGI
  • abnormal fat-soluble vitamin level / MGI
  • decreased birth weight / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • perinatal lethality, incomplete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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