B6;129S4-Ddx4tm1Embrp/Cnrm
| Status | Available to order |
| EMMA ID | EM:09169 |
| International strain name | B6;129S4-Ddx4tm1Embrp/Cnrm |
| Alternative name | Ddx4-E457Q or Mvh E457Q |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Ddx4tm1Embrp |
| Gene/Transgene symbol | Ddx4 |
Information from provider
| Provider | Ramesh PILLAI |
| Provider affiliation | EMBL Grenoble Outstation, European Molecular Biology Laboratory (EMBL) |
| Genetic information | Single amino acid point mutation of the ATPase motif (DEAD to DQAD). Presence of loxP sites allows generation of conditional knock-out line. |
| Phenotypic information | Homozygous:Heterozygous knock-out mice are already shown to display male-specific infertility (Tanaka SS, et al., The mouse homolog of Drosophila Vasa is required for the development of male germ cells. Genes Dev. 2000 Apr 1;14(7):841-53). We expect our catalytic-dead point mutation of the RNA helicase domain to display similar phenotype: males are infertile in homozygous background, while females of all backgrounds and male of heterozygous background are fertile. I will let EMMA know of the phenotype as soon as I have it in a few months.Heterozygous:Heterozygous knock-out mice are already shown to display male-specific infertility (Tanaka SS, et al., The mouse homolog of Drosophila Vasa is required for the development of male germ cells. Genes Dev. 2000 Apr 1;14(7):841-53). We expect our catalytic-dead point mutation of the RNA helicase domain to display similar phenotype: males are infertile in homozygous background, while females of all backgrounds and male of heterozygous background are fertile. I will let EMMA know of the phenotype as soon as I have it in a few months. |
| Breeding history | Founder mice were crossed with wildtype C57BL/6 females. F1 generation animals are being offered for archiving to EMMA. |
| References |
|
| Homozygous fertile | females only |
| Homozygous viable | females only |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Distinct Roles of RNA Helicases MVH and TDRD9 in PIWI Slicing-Triggered Mammalian piRNA Biogenesis and Function.;Wenda Joanna M, Homolka David, Yang Zhaolin, Spinelli Pietro, Sachidanandam Ravi, Pandey Radha Raman, Pillai Ramesh S, ;2017;Developmental cell;41;623-637.e9; 28633017