C57BL/6N-A^tm1Brd Chtop^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:09273
Citation information	RRID:IMSR_EM:09273 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-A^tm1Brd Chtop^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0635_2_D03
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Chtop^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Chtop
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0635_2_D03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal joint morphology / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

abnormal joint morphology / IMPC
preweaning lethality, complete penetrance / IMPC

MGI phenotypes (allele matching)

absent vertebrae / MGI
abnormal vertebral body morphology / MGI
rib bifurcation / MGI
rib fusion / MGI
scoliosis / MGI
double outlet right ventricle / MGI
abnormal maxilla morphology / MGI
abnormal rectum morphology / MGI
abnormal small intestine placement / MGI
abnormal salivary gland morphology / MGI
abnormal pineal gland morphology / MGI
abnormal thyroid gland morphology / MGI
absent spleen / MGI
spleen hypoplasia / MGI
abnormal forebrain morphology / MGI
abnormal choroid plexus morphology / MGI
fused dorsal root ganglion / MGI
small superior cervical ganglion / MGI
small nodose ganglion / MGI
internal hemorrhage / MGI
abnormal eye morphology / MGI
persistent truncus arteriosis / MGI
abnormal atrioventricular valve morphology / MGI
anal atresia / MGI
decreased rib number / MGI
absent olfactory bulb / MGI
thyroid hypoplasia / MGI
urinary bladder hypoplasia / MGI
abnormal eye muscle morphology / MGI
abnormal Wolffian duct morphology / MGI
diaphragmatic hernia / MGI
right aortic arch / MGI
retroesophageal right subclavian artery / MGI
abnormal adenohypophysis morphology / MGI
abnormal neurohypophysis morphology / MGI
fetal growth retardation / MGI
abnormal optic stalk morphology / MGI
abnormal optic cup morphology / MGI
absent vertebral arch / MGI
abnormal cervical atlas morphology / MGI
fusion of vertebral arches / MGI
decreased cervical vertebrae number / MGI
decreased thoracic vertebrae number / MGI
holoprosencephaly / MGI
abnormal viscerocranium morphology / MGI
arteriovenous malformation / MGI
abnormal fetal atrioventricular canal morphology / MGI
absent dorsal root ganglion / MGI
abnormal interventricular foramen morphology / MGI
abnormal hyoid bone body morphology / MGI
ostium primum atrial septal defect / MGI
common atrium / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
anomalous pulmonary venous connection / MGI
bicuspid aortic valve / MGI
abnormal hepatic portal vein morphology / MGI
fusion of atlas and occipital bones / MGI
fused right lung lobes / MGI
pelvic kidney / MGI
blind ureter / MGI
intestinal stenosis / MGI
myelocele / MGI
absent hypoglossal canal / MGI
thin hypoglossal nerve / MGI
absent hypoglossal nerve / MGI
ductus venosus stenosis / MGI
subcutaneous edema / MGI
abnormal hepatic portal vein formation / MGI
absent celiac artery / MGI
abnormal abdominal muscle morphology / MGI
abnormal azygos vein topology / MGI
abnormal vitelline vein connection / MGI
anastomosis between common carotid and vertebral artery / MGI
abnormal pancreas topology / MGI
abnormal dorsal pancreas topology / MGI
abnormal ductus venosus morphology / MGI
absent ductus venosus valve / MGI
abnormal ductus venosus valve topology / MGI
absent costovertebral joint / MGI
abnormal brachial plexus formation / MGI
abnormal olfactory bulb position / MGI
abnormal ureter topology / MGI
retro-esophageal left subclavian artery / MGI
reduced sympathetic cervical ganglion size / MGI
abnormal carotid artery origin / MGI
abnormal subclavian artery origin / MGI
abnormal vitelline vein topology / MGI
absent intrahepatic inferior vena cava segment / MGI
absent parasellar internal carotid artery / MGI
abnormal vertebral artery topology / MGI
absent extracranial vertebral artery segment / MGI
embryo cyst / MGI
heterochrony / MGI

MGI phenotypes (gene matching)

absent vertebrae / MGI
abnormal vertebral body morphology / MGI
rib bifurcation / MGI
rib fusion / MGI
scoliosis / MGI
double outlet right ventricle / MGI
abnormal maxilla morphology / MGI
abnormal rectum morphology / MGI
abnormal small intestine placement / MGI
abnormal salivary gland morphology / MGI
abnormal pineal gland morphology / MGI
abnormal thyroid gland morphology / MGI
absent spleen / MGI
spleen hypoplasia / MGI
abnormal forebrain morphology / MGI
abnormal choroid plexus morphology / MGI
fused dorsal root ganglion / MGI
small superior cervical ganglion / MGI
small nodose ganglion / MGI
internal hemorrhage / MGI
abnormal eye morphology / MGI
persistent truncus arteriosis / MGI
abnormal atrioventricular valve morphology / MGI
anal atresia / MGI
decreased rib number / MGI
absent olfactory bulb / MGI
thyroid hypoplasia / MGI
urinary bladder hypoplasia / MGI
abnormal eye muscle morphology / MGI
abnormal Wolffian duct morphology / MGI
diaphragmatic hernia / MGI
right aortic arch / MGI
retroesophageal right subclavian artery / MGI
abnormal adenohypophysis morphology / MGI
abnormal neurohypophysis morphology / MGI
fetal growth retardation / MGI
abnormal optic stalk morphology / MGI
abnormal optic cup morphology / MGI
absent vertebral arch / MGI
abnormal cervical atlas morphology / MGI
fusion of vertebral arches / MGI
decreased cervical vertebrae number / MGI
decreased thoracic vertebrae number / MGI
holoprosencephaly / MGI
abnormal viscerocranium morphology / MGI
arteriovenous malformation / MGI
abnormal fetal atrioventricular canal morphology / MGI
absent dorsal root ganglion / MGI
abnormal interventricular foramen morphology / MGI
abnormal hyoid bone body morphology / MGI
ostium primum atrial septal defect / MGI
common atrium / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
anomalous pulmonary venous connection / MGI
bicuspid aortic valve / MGI
abnormal hepatic portal vein morphology / MGI
fusion of atlas and occipital bones / MGI
fused right lung lobes / MGI
pelvic kidney / MGI
blind ureter / MGI
intestinal stenosis / MGI
myelocele / MGI
absent hypoglossal canal / MGI
thin hypoglossal nerve / MGI
absent hypoglossal nerve / MGI
ductus venosus stenosis / MGI
subcutaneous edema / MGI
abnormal hepatic portal vein formation / MGI
absent celiac artery / MGI
abnormal abdominal muscle morphology / MGI
abnormal azygos vein topology / MGI
abnormal vitelline vein connection / MGI
anastomosis between common carotid and vertebral artery / MGI
abnormal pancreas topology / MGI
abnormal dorsal pancreas topology / MGI
abnormal ductus venosus morphology / MGI
absent ductus venosus valve / MGI
abnormal ductus venosus valve topology / MGI
absent costovertebral joint / MGI
abnormal brachial plexus formation / MGI
abnormal olfactory bulb position / MGI
abnormal ureter topology / MGI
retro-esophageal left subclavian artery / MGI
reduced sympathetic cervical ganglion size / MGI
abnormal carotid artery origin / MGI
abnormal subclavian artery origin / MGI
abnormal vitelline vein topology / MGI
absent intrahepatic inferior vena cava segment / MGI
absent parasellar internal carotid artery / MGI
abnormal vertebral artery topology / MGI
absent extracranial vertebral artery segment / MGI
embryo cyst / MGI
heterochrony / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

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Material Transfer Agreement (MTA)
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Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Chtoptm1a(EUCOMM)Wtsi/WtsiH