B6;D-Tg(tetO-lacZ,-GFP/Gria2)1Rsp/Kctt

Status	Available to order
EMMA ID	EM:09283
Citation information	RRID:IMSR_EM:09283 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;D-Tg(tetO-lacZ,-GFP/Gria2)1Rsp/Kctt
Alternative name	TgTetOGFP-GluA2/1 (labname TgOCN1)
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(tetO-lacZ,-GFP/Gria2)1Rsp
Gene/Transgene symbol	Tg(tetO-lacZ,-GFP/Gria2)1Rsp

Information from provider

Provider	Rolf Sprengel
Provider affiliation	Molecular Neurobiology, Max Planck Institute for Medical Research
Genetic information	A transgene for the N-terminal GFP tagged AMPA receptor subunit GluA2 (Gria2), together with a nuclear localised lacZ reporter gene, is under the control of the tTA-dependent bidirectional synthetic promoter. Mouse lines TgTetOGFP-GluA2/1 and TgTetOGFP-GluA2/6 are derived from two different founders from the same pronucleus injection.
Phenotypic information	Homozygous: Not known Heterozygous: When activated by tTA the GFP-GluA2 transgene can partially rescue the GluA2 knock-out (Gria2-/-) mice.
Breeding history	Embryos from matings of hemizygote transgenes TgTet0GFP-GluA2 males and C57BL/6N females were harvested and 93 embryos were cryoconserved in January 2003
References	Enhanced odor discrimination and impaired olfactory memory by spatially controlled switch of AMPA receptors.;Shimshek Derya R, Bus Thorsten, Kim Jinhyun, Mihaljevic Andre, Mack Volker, Seeburg Peter H, Sprengel Rolf, Schaefer Andreas T, ;2005;PLoS biology;3;e354; 16216087
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Karolinska Institutet, Stockholm, Sweden
Animals used for archiving	heterozygous C57BL/6N males, wild-type C57BL/6N females
Stage of embryos	2-cell

Literature references

Enhanced odor discrimination and impaired olfactory memory by spatially controlled switch of AMPA receptors.;Shimshek Derya R, Bus Thorsten, Kim Jinhyun, Mihaljevic Andre, Mack Volker, Seeburg Peter H, Sprengel Rolf, Schaefer Andreas T, ;2005;PLoS biology;3;e354; 16216087

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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