C57BL/6-Tg(Myh6-Akt1*)6445Gcon/Cnrm
| Status | Available to order |
| EMMA ID | EM:09465 |
| International strain name | C57BL/6-Tg(Myh6-Akt1*)6445Gcon/Cnrm |
| Alternative name | Akt-E40K |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Myh6-Akt1*)6445Gcon |
| Gene/Transgene symbol | Tg(Myh6-Akt1*)6445Gcon |
Information from provider
| Provider | Gianluigi Condorelli |
| Provider affiliation | Humanitas |
| Genetic information | Cardiac-specific (alpha-myosin heavy chain promoter) expression of the dominant active protein kinase Akt (PKB), Akt-E40K. |
| Phenotypic information | Homozygous:Not performed.Heterozygous:Akt overexpression produced cardiac hypertrophy at the molecular and histological levels, with a significant increase in cardiomyocyte cell size and concentric left ventricular hypertrophy. Akt-transgenic mice also showed a remarkable increase in cardiac contractility compared with wild-type controls as demonstrated by the analysis of left ventricular (dP/dtmax) in an invasive hemodynamic study, although with graded dobutamine infusion, the maximum response was not different from that in controls. Diastolic function, evaluated by left ventricular dP/dtmin, was not affected at rest but was impaired during graded dobutamine infusion. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Akt induces enhanced myocardial contractility and cell size in vivo in transgenic mice.;Condorelli Gianluigi, Drusco Alessandra, Stassi Giorgio, Bellacosa Alfonso, Roncarati Roberta, Iaccarino Guido, Russo Matteo A, Gu Yusu, Dalton Nancy, Chung Clarence, Latronico Michael V G, Napoli Claudio, Sadoshima Junichi, Croce Carlo M, Ross John, ;2002;Proceedings of the National Academy of Sciences of the United States of America;99;12333-8; 12237475