STOCK Pik3ap1tm1a(KOMP)Wtsi/CipheOrl

Status

Available to order

EMMA IDEM:09618
Citation informationRRID:IMSR_EM:09618 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameSTOCK Pik3ap1tm1a(KOMP)Wtsi/CipheOrl
Alternative nameEPD0393_2_G04
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPik3ap1tm1a(KOMP)Wtsi
Gene/Transgene symbolPik3ap1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Centre d'ImmunoPhenomique - Ciphe
Provider affiliationCentre d
Genetic informationThis mouse line originates from KOMP ES clone EPD0393_2_G04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous C57BL/6NCrl males

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased neutrophil cell number / IMPC
  • increased monocyte cell number / IMPC
  • abnormal cholesterol homeostasis / IMPC
  • decreased lymphocyte cell number / IMPC
MGI phenotypes (gene matching)
  • spleen hypoplasia / MGI
  • arrested B cell differentiation / MGI
  • decreased IgM level / MGI
  • abnormal B cell differentiation / MGI
  • decreased immunoglobulin level / MGI
  • increased IgM level / MGI
  • decreased B cell number / MGI
  • decreased B cell proliferation / MGI
  • immune system phenotype / MGI
  • decreased B-1a cell number / MGI
  • increased B-1b cell number / MGI
  • decreased follicular B cell number / MGI
  • absent B-1a cells / MGI
  • decreased pre-B cell number / MGI
  • decreased IgG2b level / MGI
  • decreased IgG3 level / MGI
  • impaired humoral immune response / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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