B6.129P2-Msx1tm1Bero/Kctt

Status

Available to order

EMMA IDEM:09716
International strain nameB6.129P2-Msx1tm1Bero/Kctt
Alternative nameMsx1tm1Bero (Synonym : Msx1-nlacZ)
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolMsx1tm1Bero,
Gene/Transgene symbolMsx1

Information from provider

ProviderBenoit Robert
Provider affiliationDevelopmental and Stem Cell Biology, Institut Pasteur
Genetic informationInsertion by homologous recombination of the complete n-lacZ coding sequence into the homeobox coding sequence of Msx1 (exon 2) in frame to the open-reading-frame of the gene. The resulting mutation encodes a (nuclear) fusion protein displaying the activity of the beta-galactosidase enzyme. The Msx1 protein activity is lost (null mutation).
Phenotypic informationHomozygous:
Homozygotes display a cleft palate and tooth agenesis. The cleft palate leads to death of the new-born during the first day after birth to the impossibility for new borns to suck milk. Homozygotes also display frequently hydrocephaly due to the absence of the subcommisural organ in the fore-brain.

Heterozygous:
Heterozygotes do not display any abnormality, except hydrocephaly at a low frequency (10%).
References
  • Insertional mutation of the mouse Msx1 homeobox gene by an nlacZ reporter gene.;Houzelstein D, Cohen A, Buckingham M E, Robert B, ;1997;Mechanisms of development;65;123-33; 9256350
Homozygous fertileno
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivingheterozygous C57BL/6J

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • absent alveolar process / MGI
  • cleft chin / MGI
  • absent incisors / MGI
  • growth retardation of molars / MGI
  • shortened head / MGI
  • abnormal cranium morphology / MGI
  • short snout / MGI
  • cyanosis / MGI
  • respiratory distress / MGI
  • absent malleus processus brevis / MGI
  • abnormal palate morphology / MGI
  • absent molars / MGI
  • limbs/digits/tail phenotype / MGI
  • palatal shelf hypoplasia / MGI
  • cleft secondary palate / MGI
  • neonatal lethality, complete penetrance / MGI
  • increased neuron number / MGI
  • dilated third ventricle / MGI
  • dilated fourth ventricle / MGI
  • abnormal diencephalon morphology / MGI
  • abnormal roof plate morphology / MGI
  • hydroencephaly / MGI
  • abnormal brain morphology / MGI
  • abnormal brain commissure morphology / MGI
  • abnormal subcommissural organ morphology / MGI
  • abnormal liver development / MGI
  • exencephaly / MGI
  • abnormal embryonic tissue morphology / MGI
  • abnormal eye morphology / MGI
  • abnormal body wall morphology / MGI
  • abnormal facial morphology / MGI

Literature references

  • Insertional mutation of the mouse Msx1 homeobox gene by an nlacZ reporter gene.;Houzelstein D, Cohen A, Buckingham M E, Robert B, ;1997;Mechanisms of development;65;123-33; 9256350

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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