C57BL/6-Tg(tetO-luc,-Acot7)F26Rttr/H
| Status | Available to order |
| EMMA ID | EM:09783 |
| International strain name | C57BL/6-Tg(tetO-luc,-Acot7)F26Rttr/H |
| Alternative name | Acot7 tg |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(tetO-luc,-Acot7)F26Rttr |
| Gene/Transgene symbol | Tg(tetO-luc,-Acot7)F26Rttr |
Information from provider
| Provider | Guy Rutter |
| Provider affiliation | Section of Cell Biology, Department of Medicine, Imperial College London |
| Genetic information | Mice express Acot7 cDNA under the control of a tetracycline-inducible bidirectional promoter. Firefly luciferase is expressed simultaneously under the control of the same promoter. Insertion was via pronuclear injection into C57BL/6J pronuclei; integration was aleatory - we do not know the location in the genome. Vector used for the subcloning of the transgene incorporated on this strain: pBI-L Vector PT3069-5 GenBank Accession No.:U89934 Catalog No. 631005 |
| Phenotypic information | Homozygous:We have not studied homozygous mice. No phenotype should be observed in the absence of further breeding with animals expressing rtTA and in the presence of doxycycline.Heterozygous:When bred with animals expressing rtTA specifically in pancreatic beta cells, and upon doxycycline administration, the mice develop mild glucose intolerance, impaired insulin secretion in response to glucose and the islets present reduced levels of fatty acid-CoA. |
| Breeding history | The founder animal was first bred with C57BL/6 wild-type, and the F1 again with C57BL/6 wild-type to confirm only one insertion by qPCR. F2 was bred with littermate controls (not containing the transgene) or C57BL/6 wild-type for at least 4 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Disallowance of Acot7 in β-Cells Is Required for Normal Glucose Tolerance and Insulin Secretion.;Martinez-Sanchez Aida, Pullen Timothy J, Chabosseau Pauline, Zhang Qifeng, Haythorne Elizabeth, Cane Matthew C, Nguyen-Tu Marie-Sophie, Sayers Sophie R, Rutter Guy A, ;2016;Diabetes;65;1268-82; 26861785