StarD10-F10
| Status | Available to order |
| EMMA ID | EM:09784 |
| Citation information | RRID:IMSR_EM:09784 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | StarD10-F10 |
| Alternative name | StarD10-F10 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Unknown at present |
| Gene/Transgene symbol | mouse StarD10 |
Information from provider
| Provider | Guy Rutter |
| Provider affiliation | Medicine, Imperial College London |
| Genetic information | Insertion of a coding cassette under the control of a bidirectional tetracycline-regulated promoter driving expression of both mouse Flag-StarD10 and firefly luciferase. Vector used for the subcloning of the transgene incorporated on this strain: pBI-L Vector PT3069-5 GenBank Accession No.:U89934 Catalog No. 631005 |
| Phenotypic information | Homozygous:N/AHeterozygous:Overexpression of the transgene when crossed with mouse expressing the reverse tetracycline transactivator and given doxycycline (possibility of a tissue specific overexpression). The transgene copy number for this line was evaluated by qPCR and estimated at 25 copies ([23.88 - 25.43], n = 14). The expression level in islets was measured by Western blot after induction by 2 g/L doxycycline in drinking water and estimated to be 120% of the control expression. In vivo phenotype: The animals were crossed with RIP7-rtTA mice allowing overexpression of the transgene specifically in pancreatic beta-cells. The doxycycline was added in drinking water (2 g/L) from 5 weeks of age and intra-peritoneal glucose tolerance tests (IPGTT, 1 g/kg glucose) were performed at 8, 12 and 16-week-old. The resulting line had no differences with control mice on chow diet. This line has not yet been tested on HFD. |
| Breeding history | Founder crossed with wild-type C57BL/6J to generate F1; F1 crossed with wild-type C57BL/6J to generate F2 to ensure the stability of the line (one site of insertion only). |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |