B6.Cg-Piwil4tm1Embrp/Cnrm
| Status | Available to order |
| EMMA ID | EM:09867 |
| International strain name | B6.Cg-Piwil4tm1Embrp/Cnrm |
| Alternative name | NHA-Piwil4 knock-in mouse |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Piwil4tm1Embrp, |
| Gene/Transgene symbol | Piwil4 |
Information from provider
| Provider | Ramesh PILLAI |
| Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
| Genetic information | Piwil4 (common name: Miwi2) protein with a N-HA tag knocked-in before the ATG. The N-peptide tag is suitable for artificial tethering of the protein to a reporter with BoxB hairpins. 1X HA tag allows detection of the protein. The mouse is to be used for artificial tethering of Piwi4 to a reporter, to study role of Piwil4 in transcriptional repression via recruitment of DNA methylation. |
| Phenotypic information | Homozygous:No observable impact on viability or fertility. The mouse is a valuable tool to study small RNA-guided DNA methylation in mammals.Heterozygous:No observable impact on viability or fertility. The mouse is a valuable tool to study small RNA-guided DNA methylation in mammals. |
| Breeding history | Founder animals (generated from modified A9 cells) were crossed with siblings or with wild-type C57BL/6 mice. No observable effect on viability or fertility. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |