FVB.129P2-Igf2rtm1Dpb/Biat

Status

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EMMA IDEM:09896
Citation informationRRID:IMSR_EM:09896 

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International strain nameFVB.129P2-Igf2rtm1Dpb/Biat
Alternative nameFVB.129P2- Igf2r-IPD
Strain typeTargeted Mutant Strains : Other targeted
Allele/Transgene symbolIgf2rtm1Dpb
Gene/Transgene symbolIgf2r

Information from provider

ProviderDenise Barlow
Provider affiliationCeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
Genetic informationThe region surrounding the Igf2r promoter is deleted (4.7kb, chr17:12,767,833-12,772,512, GRCm38/mm10) leaving 92bp from the vector and a single loxP site. The construct was integrated into E14 ES cells (129P2/OlaHsd), that were used to make chimeras with C57BL/6 embryos. The mutant was then backcrossed to FVB/N for more than 50 generations.
Phenotypic informationHomozygous:
Homozygous mice are embryonic lethal between E13.5 and E18.5 due to lack of Igf2r expression.

Heterozygous:
Maternal inheritance of the IPD allele is embryonic lethal between E13.5 and E18.5 due to lack of Igf2r expression, which is only expressed from the maternal allele due to imprinted silencing of the paternal allele.
Breeding historyIPD mice were derived from 129P2 ES cells and C57BL/6 chimeras and then backcrossed to FVB/N for over 50 generations
References
  • Imprinted silencing of Slc22a2 and Slc22a3 does not need transcriptional overlap between Igf2r and Air.;Sleutels Frank, Tjon Grace, Ludwig Thomas, Barlow Denise P, ;2003;The EMBO journal;22;3696-704; 12853484
  • Airn transcriptional overlap, but not its lncRNA products, induces imprinted Igf2r silencing.;Latos Paulina A, Pauler Florian M, Koerner Martha V, Şenergin H Başak, Hudson Quanah J, Stocsits Roman R, Allhoff Wolfgang, Stricker Stefan H, Klement Ruth M, Warczok Katarzyna E, Aumayr Karin, Pasierbek Pawel, Barlow Denise P, ;2012;Science (New York, N.Y.);338;1469-72; 23239737
  • An in vitro ES cell imprinting model shows that imprinted expression of the Igf2r gene arises from an allele-specific expression bias.;Latos Paulina A, Stricker Stefan H, Steenpass Laura, Pauler Florian M, Huang Ru, Senergin Basak H, Regha Kakkad, Koerner Martha V, Warczok Katarzyna E, Unger Christine, Barlow Denise P, ;2009;Development (Cambridge, England);136;437-48; 19141673
  • Silencing and transcriptional properties of the imprinted Airn ncRNA are independent of the endogenous promoter.;Stricker Stefan H, Steenpass Laura, Pauler Florian M, Santoro Federica, Latos Paulina A, Huang Ru, Koerner Martha V, Sloane Mathew A, Warczok Katarzyna E, Barlow Denise P, ;2008;The EMBO journal;27;3116-28; 19008856
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Veterinary Medicine, Vienna, Austria
Animals used for archivingheterozygous FVB/N males

Disease and phenotype information

MGI phenotypes (gene matching)
  • enlarged heart / MGI
  • heart hyperplasia / MGI
  • abnormal interventricular septum morphology / MGI
  • abnormal craniofacial morphology / MGI
  • polydactyly / MGI
  • kinked tail / MGI
  • short tail / MGI
  • enlarged liver / MGI
  • abnormal body water content / MGI
  • abnormal uterus morphology / MGI
  • vagina atresia / MGI
  • abnormal lung morphology / MGI
  • abnormal lung development / MGI
  • increased body weight / MGI
  • increased body size / MGI
  • absent suckling reflex / MGI
  • cyanosis / MGI
  • increased embryo size / MGI
  • edema / MGI
  • reduced male fertility / MGI
  • reduced female fertility / MGI
  • female infertility / MGI
  • decreased litter size / MGI
  • respiratory failure / MGI
  • respiratory distress / MGI
  • abnormal motor capabilities/coordination/movement / MGI
  • abnormal embryonic growth/weight/body size / MGI
  • disorganized myocardium / MGI
  • abnormal tricuspid valve morphology / MGI
  • dilated heart left ventricle / MGI
  • dilated heart right ventricle / MGI
  • increased heart weight / MGI
  • thick ventricular wall / MGI
  • enlarged kidney / MGI
  • genetic imprinting / MGI
  • maternal imprinting / MGI
  • abnormal external female genitalia morphology / MGI
  • abnormal lysosome physiology / MGI
  • premature bone ossification / MGI
  • increased fetal size / MGI
  • pulmonary hyperplasia / MGI
  • enlarged placenta / MGI
  • split sternum / MGI
  • short sternum / MGI
  • enlarged lung / MGI
  • enlarged uterus / MGI
  • increased placenta weight / MGI
  • dilated heart / MGI
  • liver hyperplasia / MGI
  • increased brain size / MGI
  • abnormal myocardium layer morphology / MGI
  • homeostasis/metabolism phenotype / MGI
  • growth/size/body region phenotype / MGI
  • reproductive system phenotype / MGI
  • abnormal circulating hormone level / MGI
  • congestive heart failure / MGI
  • thin endometrium / MGI
  • abnormal bone ossification / MGI
  • decreased survivor rate / MGI
  • increased heart ventricle size / MGI
  • abnormal uterine horn morphology / MGI
  • dilated uterine horn / MGI
  • absent endometrial glands / MGI
  • increased nucleated erythrocyte cell number / MGI
  • decreased fetal weight / MGI
  • increased fetal weight / MGI
  • increased birth weight / MGI
  • heart hemorrhage / MGI
  • increased birth body size / MGI
  • increased heart right ventricle size / MGI
  • thick interventricular septum / MGI
  • mortality/aging / MGI
  • impaired branching involved in preterminal bronchiole morphogenesis / MGI
  • postnatal lethality, complete penetrance / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • neonatal lethality, complete penetrance / MGI
  • neonatal lethality, incomplete penetrance / MGI
  • perinatal lethality, incomplete penetrance / MGI
  • prenatal lethality, complete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • preweaning lethality, incomplete penetrance / MGI
  • enlarged uterine horn / MGI

Literature references

  • Imprinted silencing of Slc22a2 and Slc22a3 does not need transcriptional overlap between Igf2r and Air.;Sleutels Frank, Tjon Grace, Ludwig Thomas, Barlow Denise P, ;2003;The EMBO journal;22;3696-704; 12853484
  • Airn transcriptional overlap, but not its lncRNA products, induces imprinted Igf2r silencing.;Latos Paulina A, Pauler Florian M, Koerner Martha V, Şenergin H Başak, Hudson Quanah J, Stocsits Roman R, Allhoff Wolfgang, Stricker Stefan H, Klement Ruth M, Warczok Katarzyna E, Aumayr Karin, Pasierbek Pawel, Barlow Denise P, ;2012;Science (New York, N.Y.);338;1469-72; 23239737
  • An in vitro ES cell imprinting model shows that imprinted expression of the Igf2r gene arises from an allele-specific expression bias.;Latos Paulina A, Stricker Stefan H, Steenpass Laura, Pauler Florian M, Huang Ru, Senergin Basak H, Regha Kakkad, Koerner Martha V, Warczok Katarzyna E, Unger Christine, Barlow Denise P, ;2009;Development (Cambridge, England);136;437-48; 19141673
  • Silencing and transcriptional properties of the imprinted Airn ncRNA are independent of the endogenous promoter.;Stricker Stefan H, Steenpass Laura, Pauler Florian M, Santoro Federica, Latos Paulina A, Huang Ru, Koerner Martha V, Sloane Mathew A, Warczok Katarzyna E, Barlow Denise P, ;2008;The EMBO journal;27;3116-28; 19008856

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

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