FVB.129P2-Igf2rtm1Dpb/Biat
| Status | Available to order |
| EMMA ID | EM:09896 |
| International strain name | FVB.129P2-Igf2rtm1Dpb/Biat |
| Alternative name | FVB.129P2- Igf2r-IPD |
| Strain type | Targeted Mutant Strains : Other targeted |
| Allele/Transgene symbol | Igf2rtm1Dpb, |
| Gene/Transgene symbol | Igf2r |
Information from provider
| Provider | Denise Barlow |
| Provider affiliation | CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences |
| Genetic information | The region surrounding the Igf2r promoter is deleted (4.7kb, chr17:12,767,833-12,772,512, GRCm38/mm10) leaving 92bp from the vector and a single loxP site. The construct was integrated into E14 ES cells (129P2/OlaHsd), that were used to make chimeras with C57BL/6 embryos. The mutant was then backcrossed to FVB/N for more than 50 generations. |
| Phenotypic information | Homozygous:Homozygous mice are embryonic lethal between E13.5 and E18.5 due to lack of Igf2r expression.Heterozygous:Maternal inheritance of the IPD allele is embryonic lethal between E13.5 and E18.5 due to lack of Igf2r expression, which is only expressed from the maternal allele due to imprinted silencing of the paternal allele. |
| Breeding history | IPD mice were derived from 129P2 ES cells and C57BL/6 chimeras and then backcrossed to FVB/N for over 50 generations |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous FVB/N |
Literature references
- Imprinted silencing of Slc22a2 and Slc22a3 does not need transcriptional overlap between Igf2r and Air.;Sleutels Frank, Tjon Grace, Ludwig Thomas, Barlow Denise P, ;2003;The EMBO journal;22;3696-704; 12853484
- Airn transcriptional overlap, but not its lncRNA products, induces imprinted Igf2r silencing.;Latos Paulina A, Pauler Florian M, Koerner Martha V, Şenergin H Başak, Hudson Quanah J, Stocsits Roman R, Allhoff Wolfgang, Stricker Stefan H, Klement Ruth M, Warczok Katarzyna E, Aumayr Karin, Pasierbek Pawel, Barlow Denise P, ;2012;Science (New York, N.Y.);338;1469-72; 23239737
- An in vitro ES cell imprinting model shows that imprinted expression of the Igf2r gene arises from an allele-specific expression bias.;Latos Paulina A, Stricker Stefan H, Steenpass Laura, Pauler Florian M, Huang Ru, Senergin Basak H, Regha Kakkad, Koerner Martha V, Warczok Katarzyna E, Unger Christine, Barlow Denise P, ;2009;Development (Cambridge, England);136;437-48; 19141673
- Silencing and transcriptional properties of the imprinted Airn ncRNA are independent of the endogenous promoter.;Stricker Stefan H, Steenpass Laura, Pauler Florian M, Santoro Federica, Latos Paulina A, Huang Ru, Koerner Martha V, Sloane Mathew A, Warczok Katarzyna E, Barlow Denise P, ;2008;The EMBO journal;27;3116-28; 19008856