STOCK Pcsk1tm1a(EUCOMM)Wtsi/IcsOrl

Status

Available to order

EMMA IDEM:09998
Citation informationRRID:IMSR_EM:09998 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameSTOCK Pcsk1tm1a(EUCOMM)Wtsi/IcsOrl
Alternative nameEPD0508_1_C08
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPcsk1tm1a(EUCOMM)Wtsi
Gene/Transgene symbolPcsk1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider ICS, Institut Clinique de la Souris
Provider affiliationICS, Institut Clinique de la Souris
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0508_1_C08. For further details on the construction of this clone see the page at the IKMC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous C57BL/6NTac males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • preweaning lethality, incomplete penetrance / IMPC
  • abnormal vitreous body morphology / IMPC
  • decreased locomotor activity / IMPC
  • abnormal retina vasculature morphology / IMPC
  • abnormal freezing behavior / IMPC
  • persistence of hyaloid vascular system / IMPC
  • abnormal retina blood vessel morphology / IMPC
MGI phenotypes (gene matching)
  • increased white adipose tissue amount / MGI
  • hypoglycemia / MGI
  • enlarged spleen / MGI
  • increased body weight / MGI
  • obese / MGI
  • decreased body weight / MGI
  • polyphagia / MGI
  • chronic diarrhea / MGI
  • reduced fertility / MGI
  • abnormal spleen white pulp morphology / MGI
  • abnormal spleen marginal zone morphology / MGI
  • decreased circulating glucagon level / MGI
  • decreased circulating insulin level / MGI
  • abnormal hormone level / MGI
  • transmission ratio distortion / MGI
  • increased skeletal muscle mass / MGI
  • decreased skeletal muscle mass / MGI
  • decreased testis weight / MGI
  • decreased mean systemic arterial blood pressure / MGI
  • increased lymphocyte cell number / MGI
  • decreased T cell number / MGI
  • diarrhea / MGI
  • decreased circulating growth hormone level / MGI
  • decreased adrenocorticotropin level / MGI
  • abnormal metabolism / MGI
  • abnormal glucose tolerance / MGI
  • impaired glucose tolerance / MGI
  • insulin resistance / MGI
  • abnormal inguinal fat pad morphology / MGI
  • homeostasis/metabolism phenotype / MGI
  • increased susceptibility to diet-induced obesity / MGI
  • increased circulating leptin level / MGI
  • decreased NK T cell number / MGI
  • abnormal T-helper 1 cell differentiation / MGI
  • decreased follicular dendritic cell number / MGI
  • abnormal peritoneal macrophage morphology / MGI
  • intermingled spleen red and white pulp / MGI
  • slow postnatal weight gain / MGI
  • increased circulating tumor necrosis factor level / MGI
  • increased circulating interferon-gamma level / MGI
  • increased circulating interleukin-10 level / MGI
  • increased circulating interleukin-6 level / MGI
  • increased circulating interleukin-12 level / MGI
  • increased circulating interleukin-1 beta level / MGI
  • increased interleukin-1 beta secretion / MGI
  • increased susceptibility to endotoxin shock / MGI
  • increased sensitivity to xenobiotic induced morbidity/mortality / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • embryonic lethality before implantation, complete penetrance / MGI
  • prenatal lethality, incomplete penetrance / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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