- decreased lean body mass / IMPC
- increased total body fat amount / IMPC
- decreased bone mineral content / IMPC
- embryonic lethality prior to organogenesis / IMPC
- embryonic lethality prior to tooth bud stage / IMPC
- preweaning lethality, complete penetrance / IMPC
- decreased circulating creatinine level / IMPC
C57BL/6-Eomestm1.1Twa/Cnbc
Status | Available to order |
EMMA ID | EM:10580 |
International strain name | C57BL/6-Eomestm1.1Twa/Cnbc |
Alternative name | Eomes-GFP |
Strain type | Targeted Mutant Strains : Knock-in |
Allele/Transgene symbol | Eomestm1.1Twa |
Gene/Transgene symbol | Eomes |
Information from provider
Provider | Thierry Walzer |
Provider affiliation | Centre International de Recherche en Infectiologie (CIRI) |
Genetic information | An IRES-GFP reporter cassette was inserted in the 3' untranslated region of Eomes gene. |
Phenotypic information | Homozygous:EGFP is expressed in Eomes positive cells, without altering Eomes protein expression. Homozygous mice are normal and have a normal immune function.Heterozygous:Eomes positive cells already express high levels of EGFP |
Breeding history | ES cells (JM8) are C57BL/6N. The mice were backcrossed at least three times to C57BL/6J. |
References |
|
Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Animals used for archiving | homozygous C57BL/6J, homozygous C57BL/6J |
Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Microcephaly-polymicrogyria-corpus callosum agenesis syndrome / Orphanet_171703
IMPC phenotypes (gene matching)
MGI phenotypes (gene matching)
- absent mesoderm / MGI
- absent trophoblast giant cells / MGI
- failure of embryo implantation / MGI
- embryonic growth arrest / MGI
- no abnormal phenotype detected / MGI
- no phenotypic analysis / MGI
- abnormal egg cylinder morphology / MGI
- abnormal trophoblast layer morphology / MGI
- decreased NK cell number / MGI
- decreased memory T cell number / MGI
- absent primitive node / MGI
- embryonic lethality between implantation and placentation / MGI
- embryonic lethality before implantation, complete penetrance / MGI
- embryonic lethality between implantation and placentation, complete penetrance / MGI
- embryonic lethality between implantation and somite formation, complete penetrance / MGI
- absent trophectoderm / MGI
- abnormal primitive streak elongation / MGI
- abnormal anterior primitive streak morphology / MGI
Literature references
- T-bet and Eomes instruct the development of two distinct natural killer cell lineages in the liver and in the bone marrow.;Daussy Cécile, Faure Fabrice, Mayol Katia, Viel Sébastien, Gasteiger Georg, Charrier Emily, Bienvenu Jacques, Henry Thomas, Debien Emilie, Hasan Uzma A, Marvel Jacqueline, Yoh Keigyou, Takahashi Satoru, Prinz Immo, de Bernard Simon, Buffat Laurent, Walzer Thierry, ;2014;The Journal of experimental medicine;211;563-77; 24516120
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