B6N.129X1-Scara5tm1Ktry/Kctt

Status

Available to order

EMMA IDEM:10584
International strain nameB6N.129X1-Scara5tm1Ktry/Kctt
Alternative nameB6N.129X1-Scara5 tm1Ktry
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolScara5tm1Ktry
Gene/Transgene symbolScara5

Information from provider

ProviderKarl Tryggvason
Provider affiliationKarolinska Institutet
Additional ownerDr. David Lillicrap, Department of Pathology and Molecular Medicine, Richardson Laboratory, Queen’s University, Kingston, Ontario, Canada
Genetic informationMost of exon 3 encoding the transmembrane domain of Scara5 was deleted (chromosomal position: 65689781-65689865 bp on chromosome 14) and replaced in-frame by EGFP cassette (EGFP-polyA-Neo, Neomycin gene runs to the opposite direction with its own promoter) with the remaining intracellular sequence of Scara5.
Phenotypic informationHomozygous:
The mice are born in normal Mendelian distribution without any apparent phenotype. Their lifespan is normal, but with age one can find signs of different autoimmune diseases with inflammatory cell infiltrates in different organs such as lung, skin, liver, adipose tissue. Serum also contains high titres of antinuclear antibodies (ANA). The male mice can develop infertility with age with epididymal obstruction and cell loss in the seminiferous tubuli. This occurs in about 70 % of the male mice and can be seen around 3 months of age.

Heterozygous:
Normal
Breeding historyThe mice have been backcrossed more than 10 times with C57BL/6N mice. Currently they have been bred as full knockouts for 2 generations.
References
  • Progressive reactive lymphoid connective tissue disease and development of autoantibodies in scavenger receptor A5-deficient mice.;Ojala Juha Risto Matias, Pikkarainen Timo, Elmberger Göran, Tryggvason Karl, ;2013;The American journal of pathology;182;1681-95; 23499552
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivinghomozygous C57BL/6N

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal testis morphology / MGI
  • liver inflammation / MGI
  • lung inflammation / MGI
  • interstitial pneumonia / MGI
  • reduced male fertility / MGI
  • increased anti-nuclear antigen antibody level / MGI
  • skin inflammation / MGI
  • urinary bladder inflammation / MGI
  • adipose tissue inflammation / MGI
MGI phenotypes (gene matching)
  • abnormal testis morphology / MGI
  • liver inflammation / MGI
  • lung inflammation / MGI
  • interstitial pneumonia / MGI
  • reduced male fertility / MGI
  • increased anti-nuclear antigen antibody level / MGI
  • skin inflammation / MGI
  • urinary bladder inflammation / MGI
  • adipose tissue inflammation / MGI

Literature references

  • Progressive reactive lymphoid connective tissue disease and development of autoantibodies in scavenger receptor A5-deficient mice.;Ojala Juha Risto Matias, Pikkarainen Timo, Elmberger Göran, Tryggvason Karl, ;2013;The American journal of pathology;182;1681-95; 23499552

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).