IMPC phenotypes (gene matching)
B6.Cg-Setdb2tm1Desr/Biat
| Status | Available to order |
| EMMA ID | EM:11045 |
| International strain name | B6.Cg-Setdb2tm1Desr/Biat |
| Alternative name | Setdb2_ex3GT/GT |
| Strain type | Gene-trap |
| Allele/Transgene symbol | Setdb2tm1Desr |
| Gene/Transgene symbol | Setdb2 |
Information from provider
| Provider | David Symer |
| Provider affiliation | Dept. of Lymphoma & Myeloma, Univ. Texas MD Anderson Cancer Center |
| Genetic information | To generate Setdb2GT/GT mice, the targeting vector pEKF106 was made by recombination using Lambda RED system in E. coli strain EL35047. A genomic PAC library (i.e. from the 129/SvEvTac fBr genetic background, RPCI mouse PAC library 21; MRC Geneservice) was screened for the full-length Setdb2 gene using specific cDNA probes. A positive PAC clone, RP21-498J23, was used for recovery of flanking genomic sequences. We generated a 2.3kb genomic fragment introducing an EcoRV restriction site and loxP-neo using plasmid pLMJ237, for insertion downstream of exon 3. Neo was removed by arabinose induction of Cre recombinase. Similarly, a 2.9kb genomic fragment harbouring the frt-loxP flanked gene trap cassette and a heterologous BamHI restriction site was generated by recombination using pLTM330. The gene trap cassette contains a pGK/EM7 dual promoter, a strong splice acceptor site from the engrailed 2 gene and Neo reporter gene, followed by a strong polyadenylation signal (Supplementary Fig. 3A). NotI linearized targeting vector DNA was transfected into v6.4 embryonic stem (ES) cells, a hybrid of the C57BL/6J × 129/SvJae lineages. Transfected ES cells were selected for neomycin resistance with G418 and positive clones were tested by Southern blot analysis of EcoRV-digested DNA. Positive ES cell clones were microinjected into blastulae and transferred to pseudopregnant female mice, following standard methods. |
| Phenotypic information | Homozygous:altered immune responseHeterozygous:unknown |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
Disease and phenotype information
Literature references
- The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection.;Schliehe Christopher, Flynn Elizabeth K, Vilagos Bojan, Richson Udochuku, Swaminanthan Savitha, Bosnjak Berislav, Bauer Lisa, Kandasamy Richard K, Griesshammer Isabel M, Kosack Lindsay, Schmitz Frank, Litvak Vladimir, Sissons James, Lercher Alexander, Bhattacharya Anannya, Khamina Kseniya, Trivett Anna L, Tessarollo Lino, Mesteri Ildiko, Hladik Anastasiya, Merkler Doron, Kubicek Stefan, Knapp Sylvia, Epstein Michelle M, Symer David E, Aderem Alan, Bergthaler Andreas, ;2015;Nature immunology;16;67-74; 25419628