| Status | Available to order |
| EMMA ID | EM:00602 |
| International strain name | B6.129S1/Sv-Bcl2l11tm1.1Ast/Kieg |
| Alternative name | bim-/- |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Bcl2l11tm1.1Ast |
| Gene/Transgene symbol | Bcl2l11 |
| Provider | Andreas Strasser |
| Provider affiliation | Department of Medicine, Clinical Immunology and Allergy |
| Genetic information | In the targeting vector, prepared from 129/SvJ DNA, the 110 bp BH3-containing exon is replaced by the PGKneo expression cassette, flanked by loxP sites. Splicing of the remaining Bcl2l11 (bim) exons will produce a frameshift, which introduces a stop codon 12 bp downstream. Linearized targeting vector (30 mg) was electroporated into W9.5 ES cells. Hind III-digested DNA from 133 G418-resistant colonies was screened by Southern blotting with a 3-kb external probe (1.7 kb + 1.3 kb Bgl II fragments), for the 8-kb Hind III fragment. Two independent ES clones bearing a single targeted copy of the Bcl2l11 locus were microinjected into C57BL/6 blastocysts. Chimeric agouti-colored offspring were backcrossed to C57BL/6 mice or C57BL/6 cre recombinase-deleter mice. |
| Phenotypic information | Elevated leukocyte levels. Perturbed thymic T cell development. With age, Bcl2l11 (bim) deficiency leads to progressive lymphadenopathy and systemic autoimmune disease with elevated Ig levels, and often with enlarged spleen. Many older mice develop kidney disease due to immune complexes. Some of the bim-/- female mice have imperforated vagina and the bim-/- females that do get offsprings are bad mothers. Therefore female bim -/+ are used for breeding. |
| Breeding history | Bcl2l11-deficient mice (266/266 del) were backcrossed more than 12 generations to C57BL/6 background and then bred by brother x sister mating. |
| References |
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| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
