IMPC phenotypes (gene matching)
B6.129P2-Ifitm1tm1.1Ieg/Ieg
| Status | Available to order |
| EMMA ID | EM:06123 |
| International strain name | B6.129P2-Ifitm1tm1.1Ieg/Ieg |
| Alternative name | Ifitm1tm1IEG |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Ifitm1tm1.1Ieg |
| Gene/Transgene symbol | Ifitm1 |
Information from provider
| Provider | Johannes Beckers |
| Provider affiliation | Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH) |
| Additional owner | respectively, Helmholtz Zentrum Muenchen GmbH |
| Genetic information | For the generation of Ifitm1 knock-out mutant mice we designed a targeting vector replacing the entire coding region of Ifitm1 by a lacZ reporter gene. We designed a fusion PCR to insert the lacZ gene (pSV-beta-Galactosidase Control Vector, Promega) in frame with the transcription start of the Ifitm1 gene. The lacZ gene was followed by a bGHpA site and a neo cassette flanked by loxP sites under the control of a PGK/EM7 promoter for positive selection. For negative selection a diphtheria toxin (DT) cassette (pKO SelectDT, Stratagene) was introduced into the bacterial backbone of the pBluescript-II-KS+ vector. Targeted (E14) ES cell clones were used for injection into C57BL/6J blastocysts and embryo transfer into CD1 foster females. Male chimeras were bred to C57BL/6J cre/+ females to obtain germ line transmission and removal of the positive selection. The offspring was screened for the Ifitm1 knock-out allele and heterozygous Ifitm-tm1IEG/+ siblings were mated to establish the Ifitm1 knock-out line. |
| Phenotypic information | Based on coincidence of gene expression data, the identification of cis-regulatory response elements, as well as in vitro and knock-down studies, Ifitm1 was previously suggested to be required for germ cell migration, embryonic patterning, and interferon mediated immune response. We generated a mouse model lacking the Ifitm1 gene and found that it is not necessary for any of the anticipated functions. Instead the gene is selectively expressed in the bronchial epithelium of adult mice. In addition, we find strong expression of the human IFITM1 gene in epithelial derived non-small cell lung carcinomas. |
| Breeding history | The Ifitm1-tm1IEG has been maintained on the C57BL/6J background for more than 8 generations. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
Disease and phenotype information
MGI phenotypes (allele matching)
- decreased granulocyte number / MGI
- impaired balance / MGI
- increased IgE level / MGI
- decreased circulating corticosterone level / MGI
- increased liver weight / MGI
- decreased T cell number / MGI
- abnormal circulating testosterone level / MGI
- increased oxygen consumption / MGI
- embryo phenotype / MGI
- immune system phenotype / MGI
- reproductive system phenotype / MGI
- skeleton phenotype / MGI
MGI phenotypes (gene matching)
- decreased granulocyte number / MGI
- impaired balance / MGI
- increased IgE level / MGI
- decreased circulating corticosterone level / MGI
- increased liver weight / MGI
- enhanced coordination / MGI
- decreased T cell number / MGI
- abnormal circulating testosterone level / MGI
- increased oxygen consumption / MGI
- embryo phenotype / MGI
- immune system phenotype / MGI
- reproductive system phenotype / MGI
- skeleton phenotype / MGI
Literature references
- In vivo functional requirement of the mouse Ifitm1 gene for germ cell development, interferon mediated immune response and somitogenesis.;Klymiuk Ingeborg, Kenner Lukas, Adler Thure, Busch Dirk H, Boersma Auke, Irmler Martin, Fridrich Barbara, Gailus-Durner Valérie, Fuchs Helmut, Leitner Nicole, Müller Mathias, Kühn Ralf, Schlederer Michaela, Treise Irina, de Angelis Martin Hrabě, Beckers Johannes, ;2012;PloS one;7;e44609; 23115618